Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstrasse 11a, 07745, Jena, Germany.
Junior Research Group Chemistry of Microbial Communication, Jena, Germany.
Angew Chem Int Ed Engl. 2016 Jul 25;55(31):8944-7. doi: 10.1002/anie.201603312. Epub 2016 Jun 13.
Bacterial defense mechanisms have evolved to protect bacteria against predation by nematodes, predatory bacteria, or amoebae. We identified novel bacterial alkaloids (pyreudiones A-D) that protect the producer, Pseudomonas fluorescens HKI0770, against amoebal predation. Isolation, structure elucidation, total synthesis, and a proposed biosynthetic pathway for these structures are presented. The generation of P. fluorescens gene-deletion mutants unable to produce pyreudiones rendered the bacterium edible to a variety of soil-dwelling amoebae.
细菌防御机制的进化是为了保护细菌免受线虫、捕食性细菌或变形虫的捕食。我们鉴定了新型细菌生物碱（吡雷酮 A-D），它们可以保护产生菌荧光假单胞菌 HKI0770 免受变形虫的捕食。本文介绍了这些结构的分离、结构解析、全合成以及一个可能的生物合成途径。生成的荧光假单胞菌基因缺失突变体无法产生吡雷酮，使细菌成为各种土壤变形虫的可食用食物。
