Götze Sebastian, Herbst-Irmer Regine, Klapper Martin, Görls Helmar, Schneider Kilian R A, Barnett Robert, Burks Thomas, Neu Ursula, Stallforth Pierre
Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI) , Junior Research Group Chemistry of Microbial Communication, Beutenbergstraße 11a, 07745 Jena, Germany.
Universität Göttingen, Insitute of Inorganic Chemistry , Tammannstraße 4, 37077 Göttingen, Germany.
ACS Chem Biol. 2017 Oct 20;12(10):2498-2502. doi: 10.1021/acschembio.7b00589. Epub 2017 Sep 1.
The class of cyclic lipopeptide natural products consists of compounds with a diverse range of bioactivities. In this study, we elucidated the structure of the cyclic lipopeptide anikasin using X-ray crystallography, analyzed its biosynthetic gene cluster, and investigated its natural role in the interaction between the producer strain Pseudomonas fluorescens HKI0770 and protozoal predators. These results led to the conclusion that anikasin has dual functionality enabling swarming motility and acting as a niche amoebicide, which effectively inhibits the social amoeba Polysphondylium violaceum and protects the producer strain from protozoal grazing.
环状脂肽类天然产物由具有多种生物活性的化合物组成。在本研究中，我们利用X射线晶体学阐明了环状脂肽阿尼卡辛的结构，分析了其生物合成基因簇，并研究了其在产生菌荧光假单胞菌HKI0770与原生动物捕食者相互作用中的天然作用。这些结果得出结论，阿尼卡辛具有双重功能，既能促进群体游动，又能作为一种生态位杀阿米巴剂，有效抑制社会性变形虫紫多头绒泡菌，并保护产生菌免受原生动物捕食。
