Lee Kyung-Jo, Lee Mi-Ae, Hwang Won, Park Hana, Lee Kyu-Ho
a Department of Life Science , Sogang University , Seoul , Republic of Korea.
Biofouling. 2016 Aug;32(7):711-23. doi: 10.1080/08927014.2016.1193595.
The extracellular polysaccharides of Vibrio vulnificus play different roles during biofilm development. Among them, the effect of lipopolysaccharide (LPS), which is crucial for bacterial adherence to surfaces during the initial stage of biofilm formation, on the formation process was examined using various types of LPS extracts. Exogenously added LPS strongly inhibited biofilm formation in a dose-dependent manner. In addition, the exogenous addition of a deacylated form of LPS (dLPS) also inhibited biofilm formation. However, an LPS fraction extracted from a mutant not able to produce O-antigen polysaccharides (O-Ag) did not have an inhibitory effect. Furthermore, biofilm formation by several Gram-negative bacteria was inhibited by dLPS addition. In contrast, biofilm formation by Gram-positive bacteria was not influenced by dLPS but was affected by lipoteichoic acid. Therefore, this study demonstrates that O-Ag in LPS is important for inhibiting biofilm formation and may serve an efficient anti-biofilm agent specific for Gram-negative bacteria.
创伤弧菌的胞外多糖在生物膜形成过程中发挥着不同作用。其中，利用多种脂多糖（LPS）提取物研究了脂多糖在生物膜形成初始阶段对细菌黏附于表面至关重要的作用及其对形成过程的影响。外源添加的脂多糖以剂量依赖的方式强烈抑制生物膜形成。此外，外源添加去酰化形式的脂多糖（dLPS）也抑制生物膜形成。然而，从无法产生O抗原多糖（O-Ag）的突变体中提取的脂多糖组分没有抑制作用。此外，添加dLPS可抑制几种革兰氏阴性菌的生物膜形成。相比之下，革兰氏阳性菌的生物膜形成不受dLPS影响，但受脂磷壁酸影响。因此，本研究表明脂多糖中的O-Ag对抑制生物膜形成很重要，可能是一种对革兰氏阴性菌有效的抗生物膜剂。
