Ma Guoxiang, Ding Yu, Wu Qingping, Zhang Jumei, Liu Ming, Wang Zhi, Wang Zimeng, Wu Shi, Yang Xiaojuan, Li Ying, Wei Xianhu, Wang Juan
College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Microorganisms. 2022 Nov 29;10(12):2357. doi: 10.3390/microorganisms10122357.
() is an important food-borne and zoonotic pathogen. It can form biofilm on the surface of food, increasing the risk to food safety. Generally, outer membrane vesicles (OMVs) are spherical nanostructures secreted by Gram-negative bacteria during growth. They play a role in biological processes because they contain biologically active molecules. Several studies have reported that OMVs secreted by various bacteria are associated with the formation of biofilms. However, the interactions between OMVs and biofilm are unknown. This study aims to investigate the effect of OMVs on biofilm formation. Firstly, OMVs were extracted from Y1083, which has a strong biofilm-forming ability, at 15 °C, 28 °C and 37 °C and then characterized. The characterization results showed differences in the yield and protein content of three types of OMVs. Next, by co-culturing the OMVs with , it was observed that the OMVs inhibited the initial stage of biofilm formation but did not affect the growth of . Furthermore, biofilm formation by and were also inhibited by OMVs. Subsequently, it was proved that lipopolysaccharides (LPS) in OMVs inhibited biofilm formation., The proteins, DNA or RNA in OMVs could not inhibit biofilm formation. Bacterial motility and the expression of the biofilm-related genes , and were inhibited by LPS. LPS demonstrated good anti-biofilm activity against various bacteria. This study provides a new approach to the prevention and control of pathogenic bacterial biofilm.
（）是一种重要的食源性病原体和人畜共患病原体。它可在食品表面形成生物膜，增加食品安全风险。一般来说，外膜囊泡（OMVs）是革兰氏阴性菌在生长过程中分泌的球形纳米结构。它们在生物过程中发挥作用，因为它们含有生物活性分子。多项研究报道，各种细菌分泌的OMVs与生物膜的形成有关。然而，OMVs与生物膜之间的相互作用尚不清楚。本研究旨在探讨OMVs对生物膜形成的影响。首先，从具有较强生物膜形成能力的Y1083中在15℃、28℃和37℃下提取OMVs，然后进行表征。表征结果显示三种类型的OMVs在产量和蛋白质含量上存在差异。接下来，通过将OMVs与 共培养，观察到OMVs抑制了生物膜形成的初始阶段，但不影响 的生长。此外，OMVs也抑制了 和 的生物膜形成。随后，证明OMVs中的脂多糖（LPS）抑制生物膜形成。OMVs中的蛋白质、DNA或RNA不能抑制生物膜形成。LPS抑制了细菌的运动性以及生物膜相关基因 、 和 的表达。LPS对多种细菌表现出良好的抗生物膜活性。本研究为致病性细菌生物膜的预防和控制提供了一种新方法。
