Sokolove P M, Kester M B
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
Anal Biochem. 1989 Mar;177(2):402-6. doi: 10.1016/0003-2697(89)90074-2.
Arsenazo III-loaded liposomes are in wide use as model systems in the study of Ca2+ transport. The most sophisticated method for quantitation of Ca2+ uptake [E. B. Smaal et al. (1985) Biochim. Biophys. Acta 816, 418] utilizes the absorbance changes (650-700 nm) elicited by sequential additions of EDTA and A23187 to distinguish Ca2+-Arsenazo complexes which are outside and inside the liposomes. In this paper, the analytical approach of Smaal and co-workers is reevaluated and a straightforward treatment that allows calculation both of the concentration of Ca2+ inside liposomes and of total Ca2+ uptake (in moles/mole phospholipid) is developed.
负载偶氮胂III的脂质体作为研究Ca2+转运的模型系统被广泛应用。最复杂的Ca2+摄取定量方法[E. B. Smaal等人（1985年），《生物化学与生物物理学报》816卷，418页]利用依次添加EDTA和A23187引发的吸光度变化（650 - 700纳米）来区分脂质体外部和内部的Ca2+ - 偶氮胂复合物。本文重新评估了Smaal及其同事的分析方法，并开发了一种直接的处理方法，该方法能够计算脂质体内Ca2+的浓度以及总Ca2+摄取量（以摩尔/摩尔磷脂计）。
