一种间接测定植物及某些生物材料中痕量结合态硒代蛋氨酸的方法。

A method for the indirect determination of trace bound selenomethionine in plants and some biological materials.

作者信息

Ouyang Z, Wu J A, Xie L Q

机构信息

Department of Chemistry, Jinan University, Guangzhou, People's Republic of China.

出版信息

Anal Biochem. 1989 Apr;178(1):77-81. doi: 10.1016/0003-2697(89)90359-x.

DOI:10.1016/0003-2697(89)90359-x

PMID:2729582

Abstract

An indirect method for the determination of trace bound selenomethionine (SeMet) has been developed. SeMet reacts with cyanogen bromide (CNBr) quantitatively in the presence of SnCl2 to form CH3SeCN, and after extraction with CHCl3 is acid-digested to form Se(IV). Selenium(IV) reacts with 4-nitro-o-phenylenediamine reagent to form 5-NO2-piazselenol which is then determined by gas chromatography equipped with electron capture detector. The sensitivity of this method (CNBr-piazselenol-GC method) is 6 ng SeMet/g of sample. Trace-bound SeMet in plants and some biological materials has been successfully determined by this method and its content has been compared with the total selenium in the sample.

摘要

已开发出一种测定痕量结合态硒代蛋氨酸（SeMet）的间接方法。在SnCl2存在下，SeMet与溴化氰（CNBr）定量反应生成CH3SeCN，用CHCl3萃取后经酸消解形成Se(IV)。硒(IV)与4-硝基邻苯二胺试剂反应生成5-NO2-硒代吡唑，然后用配备电子捕获检测器的气相色谱法进行测定。该方法（CNBr-硒代吡唑-GC法）的灵敏度为6 ng SeMet/g样品。用该方法已成功测定了植物和一些生物材料中的痕量结合态SeMet，并将其含量与样品中的总硒含量进行了比较。