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The determination of selenomethionine in selenium yeast by cyanogen bromide gas chromatography.

作者信息

Zheng O Y, Wu J

机构信息

Department of Chemistry, Jinan University, Guangzhou, People's Republic of China.

出版信息

Biomed Chromatogr. 1988 Nov;2(6):258-9. doi: 10.1002/bmc.1130020607.

DOI:10.1002/bmc.1130020607
PMID:3233396
Abstract

A new method for the determination of bonded selenomethionine in selenium yeast by gas chromatography is established. Bonded selenomethionine is rapidly and precisely determined by measuring CH3SeCN released during the reaction of selenoprotein with CNBr (CNBr-GC method). Conditions for the reaction and chromatography are described. The results of CNBr-GC are compared with those obtained by acid hydrolysis/ion exchange chromatography. This new method has the advantage of being accurate, sensitive and selective.

摘要

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