Okeke C N, Gugnani H C
Department of Microbiology, University of Nigeria, Nsukka.
Antonie Van Leeuwenhoek. 1989 Apr;55(4):313-24. doi: 10.1007/BF00398510.
Lipase activity was demonstrable titrimetrically in the culture filtrates of Fonsecaea pedrosoi and Phialophora verrucosa on the 6th day of incubation reaching a peak on the 15th and 12th days respectively for the two fungi. Purified lipases of F. pedrosoi and P. verrucosa, with specific activities of 36.0 and 39.4 fold increase respectively, were obtained by cold acetone extraction, gel filtration followed by ion exchange chromatography. The lipases had the same optimum pH (5.5) and temperature (35 degrees C). The molecular weights of the lipases of F. pedrosoi and P. verrucosa, estimated by gel filtration on Sephadex G-100, were 25,000 and 20,000, respectively and the enzymes showed broad substrate specificity. The possible role of lipase in the pathogenesis of infection caused by the fungi is discussed.
在孵育的第6天，用滴定法可在佩德罗分支孢子菌和疣状瓶霉的培养滤液中检测到脂肪酶活性，这两种真菌分别在第15天和第12天达到峰值。通过冷丙酮萃取、凝胶过滤然后离子交换色谱法，获得了佩德罗分支孢子菌和疣状瓶霉的纯化脂肪酶，其比活性分别提高了36.0倍和39.4倍。这些脂肪酶具有相同的最适pH值（5.5）和温度（35摄氏度）。通过在葡聚糖凝胶G - 100上进行凝胶过滤估算，佩德罗分支孢子菌和疣状瓶霉脂肪酶的分子量分别为25,000和20,000，并且这些酶表现出广泛的底物特异性。文中讨论了脂肪酶在真菌引起的感染发病机制中的可能作用。
