Zhang Xiao-Wei, Li Si-Yu, Zhang Ling-Ling, Yang Qiang, Jiang Qian-Tao, Ma Jian, Qi Peng-Fei, Li Wei, Chen Guo-Yue, Lan Xiu-Jin, Deng Mei, Lu Zhen-Xiang, Liu Chunji, Wei Yu-Ming, Zheng You-Liang
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
b Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada.
Genome. 2016 Jul;59(7):501-7. doi: 10.1139/gen-2016-0007. Epub 2016 May 11.
ADP-glucose pyrophosphorylase (AGP), which consists of two large subunits (AGP-L) and two small subunits (AGP-S), controls the rate-limiting step in the starch biosynthetic pathway. In this study, a full-length open reading frame (ORF) of AGP-L gene (named as Agp2) in wheat and a series of Agp2 gene sequences in wheat relatives were isolated. The coding region of Agp2 contained 15 exons and 14 introns including a full-length ORF of 1566 nucleotides, and the deduced protein contained 522 amino acids (57.8 kDa). Generally, the phylogenetic tree of Agp2 indicated that sequences from A- and D-genome donor species were most similar to each other and sequences from B-genome donor species contained more variation. Starch accumulation and Agp2 expression in wheat grains reached their peak at 21 and 15 days post anthesis (DPA), respectively.
ADP-葡萄糖焦磷酸化酶（AGP）由两个大亚基（AGP-L）和两个小亚基（AGP-S）组成，它控制着淀粉生物合成途径中的限速步骤。在本研究中，从小麦中分离出AGP-L基因（命名为Agp2）的全长开放阅读框（ORF）以及小麦近缘种中的一系列Agp2基因序列。Agp2的编码区包含15个外显子和14个内含子，其中包括一个1566个核苷酸的全长ORF，推导的蛋白质含有522个氨基酸（57.8 kDa）。一般来说，Agp2的系统发育树表明，来自A基因组和D基因组供体物种的序列彼此最相似，而来自B基因组供体物种的序列具有更多变异。小麦籽粒中的淀粉积累和Agp2表达分别在开花后21天和15天达到峰值。
