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钴胺素失活大鼠中5-甲基四氢叶酸的氧化作用

Oxidation of 5-methyltetrahydrofolate in cobalamin-inactivated rats.

作者信息

Lumb M, Deacon R, Perry J, Chanarin I

机构信息

M.R.C. Clinical Research Centre, Northwick Park Hospital, Harrow, Middx., U.K.

出版信息

Biochem J. 1989 Mar 15;258(3):907-10. doi: 10.1042/bj2580907.

DOI:10.1042/bj2580907
PMID:2730574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1138450/
Abstract

Rats were exposed to nitrous oxide, which inactivates cob(I)alamin (Cbl). As in air-breathing rats methionine administration led to the conversion of hepatic 5-methyltetrahydrofolate (MeH4 folate) into formyltetrahydrofolate. The recovery of MeH4 folate levels in liver after its oxidation initiated by methionine was noted and the rate compared with that for air-breathing rats. Oxidation of MeH4 folate was less complete and occurred more slowly in Cbl-inactivated rats as compared with controls. However, recovery of MeH4 folate levels was more rapid in Cbl inactivation. S-Adenosylmethionine did not produce a significant change in MeH4 folate levels in Cbl-inactivated rats, whereas it did so in air-breathing animals.

摘要

将大鼠暴露于能使钴胺素（Cbl）失活的一氧化二氮中。与呼吸空气的大鼠一样，给予蛋氨酸会导致肝脏中的5-甲基四氢叶酸（MeH4叶酸）转化为甲酰四氢叶酸。记录了蛋氨酸引发的氧化作用后肝脏中MeH4叶酸水平的恢复情况，并将其速率与呼吸空气的大鼠进行比较。与对照组相比，Cbl失活的大鼠中MeH4叶酸的氧化不太完全且发生得更慢。然而，在Cbl失活的情况下，MeH4叶酸水平的恢复更快。S-腺苷甲硫氨酸在Cbl失活的大鼠中未使MeH4叶酸水平产生显著变化，而在呼吸空气的动物中则会使其产生变化。

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本文引用的文献

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Interrelations of vitamin B12 and folic acid metabolism: folic acid clearance studies.维生素B12与叶酸代谢的相互关系：叶酸清除率研究
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Urinary folate loss following inactivation of vitamin B12 by nitrous oxide in rats.大鼠中氧化亚氮使维生素B12失活后尿中叶酸的流失。
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Folate pentaglutamate and folate hexaglutamate mediated one-carbon metabolism.叶酸五谷氨酸和叶酸六谷氨酸介导一碳代谢。
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Chronic cobalamin inactivation impairs folate polyglutamate synthesis in the rat.慢性钴胺素失活会损害大鼠体内叶酸多聚谷氨酸的合成。
J Clin Invest. 1983 May;71(5):1183-90. doi: 10.1172/jci110867.
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Mammalian methylenetetrahydrofolate reductase. Partial purification, properties, and inhibition by S-adenosylmethionine.哺乳动物亚甲基四氢叶酸还原酶。部分纯化、性质及受S-腺苷甲硫氨酸的抑制作用。
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Turnover of the methyl moiety of 5-methyltetrahydropteroylglutamic acid in the cobalamin-inactivated rat.钴胺素失活大鼠体内5-甲基四氢蝶酰谷氨酸甲基部分的周转
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Examination of the role of methylenetetrahydrofolate reductase in incorporation of methyltetrahydrofolate into cellular metabolism.亚甲基四氢叶酸还原酶在将甲基四氢叶酸纳入细胞代谢过程中的作用研究。
FASEB J. 1988 Jan;2(1):42-7. doi: 10.1096/fasebj.2.1.3335280.
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In vivo oxidation of the methyl group of hepatic 5-methyltetrahydrofolate.肝脏5-甲基四氢叶酸甲基基团的体内氧化。
J Clin Pathol. 1988 Nov;41(11):1158-62. doi: 10.1136/jcp.41.11.1158.
9
Folate-dependent 1-carbon transfer to biogenic amines mediated by methylenetetrahydrofolate reductase.由亚甲基四氢叶酸还原酶介导的依赖叶酸的一碳转移至生物胺过程。
Nature. 1975 Nov 13;258(5531):173-4. doi: 10.1038/258173a0.
10
5-Methyltetrahydrofolate aromatic alkylamine N-methyltransferase: an artefact of 5,10-methylenetetrahydrofolate reductase activity.5-甲基四氢叶酸芳香烷基胺N-甲基转移酶：5,10-亚甲基四氢叶酸还原酶活性的一种假象。
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