Mandal C, Nandi U S
Chem Biol Interact. 1978 Apr;21(1):125-34. doi: 10.1016/0009-2797(78)90073-x.
The kinetics of the interaction of Au(III) with whole yeast RNA has been studied using UV-spectrophotometry. The reaction is second order with respect to the nucleotide unit of RNA and first order with respect to Au(III) in the respective stoichiometry of 2 : 1. The effects of initial composition, temperature, ionic strength, pH and chloride ion on the kinetics have been studied. Activation energy is found to be 11.5 kcal/mol. Effect of ionic strength indicates that both the positively charged and neutral species of Au(III) take part in the rate limiting step, the former being dominant at low ionic strength. A plausible mechanism has been proposed which involves the interaction of two nucleotide units of RNA with one species of Au(III) in the rate limiting step.
采用紫外分光光度法研究了金（III）与全酵母RNA相互作用的动力学。该反应对于RNA的核苷酸单元为二级反应，对于金（III）为一级反应，各自的化学计量比为2:1。研究了初始组成、温度、离子强度、pH值和氯离子对动力学的影响。发现活化能为11.5千卡/摩尔。离子强度的影响表明，金（III）的带正电荷和中性物种都参与了速率决定步骤，前者在低离子强度下占主导地位。提出了一种合理的机制，该机制涉及在速率决定步骤中RNA的两个核苷酸单元与一种金（III）物种的相互作用。
