Ratnasari Neneng, Bayupurnama Putut, Maduseno Sutanto, Indrarti Fahmi, Triwikatmani Catharina, Harijadi Achmad, Nurdjanah Siti
Department of Internal Medicine, Dr. Sardjito General Hospital, Gadjah Mada University, Yogyakarta, Indonesia.
Department of Pathology, School of Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
Acta Med Iran. 2016 Jun;54(6):359-65.
Local inflammatory processes in the gastric mucosa are followed by extensive immune cell infiltration, resulting in chronic active gastritis characterized by a marked infiltration of T(h)1 cytokine-producing CD4+ and CD8+T-cells Objective. To investigate the correlation between CD4+/CD8+ T-cells in gastric mucosa with endoscopic appearance in chronic gastritis with or without H.pylori infection. Prospective, cross sectional study is performed in a chronic dyspepsia population in July-November 2009 at Dr. Sardjito General Hospital Yogyakarta, Indonesia. The update Sydney system was used to analyze the gastroscopy appearance. Biopsy specimens were stained with HE-stain and IHC-stain. Data were analyzed by t-test, Mann-Whitney and Spearman correlation test. Number of 88 consecutive subjects are enrolled the study (50% male; 50% female), age 46±15 years; 25% H.pylori positive. The expression of CD4+ and CD8+ were higher in H.pylori negative subjects, but only the CD4+ was significant (P=0.011). A significant correlation was found between CD4+ and CD8+ in both subjects (r(Hp+)=0.62 and r(Hp-)=0.68; P<0.05). The expression of CD4+ and CD8+ in H.pylori positive showed a significant correlation with gastric lesions (r(CD4+)=-0.60; r(CD8+)=-0.42 ; P<0.05), only erosion showed a significant difference in both subjects. A positive correlation was found between CD4+ and CD8+ infiltration in both subjects with or without H.pylori infection, and a negative correlation was only found between gastric lesion with CD4+ and CD8+ infiltration in H.pylori subject.
胃黏膜局部炎症过程之后会出现广泛的免疫细胞浸润，从而导致慢性活动性胃炎，其特征是产生Th1细胞因子的CD4+和CD8+T细胞显著浸润。目的：研究慢性胃炎伴或不伴幽门螺杆菌感染时胃黏膜中CD4+/CD8+T细胞与内镜表现之间的相关性。2009年7月至11月在印度尼西亚日惹市萨迪托综合医院对慢性消化不良人群进行了前瞻性横断面研究。采用更新的悉尼系统分析胃镜检查结果。活检标本进行苏木精-伊红染色（HE染色）和免疫组化染色（IHC染色）。数据采用t检验、曼-惠特尼检验和斯皮尔曼相关性检验进行分析。连续88名受试者纳入研究（50%为男性；50%为女性），年龄46±15岁；25%幽门螺杆菌阳性。幽门螺杆菌阴性受试者中CD4+和CD8+的表达较高，但只有CD4+具有显著性差异（P=0.011）。在两组受试者中均发现CD4+和CD8+之间存在显著相关性（r（Hp+）=0.62，r（Hp-）=0.68；P<0.05）。幽门螺杆菌阳性受试者中CD4+和CD8+的表达与胃部病变显著相关（r（CD4+）=-0.60；r（CD8+）=-0.42；P<0.05），仅糜烂在两组受试者中显示出显著差异。在伴或不伴幽门螺杆菌感染的两组受试者中，CD4+和CD8+浸润之间均呈正相关，而在幽门螺杆菌感染受试者中，仅胃部病变与CD4+和CD8+浸润之间呈负相关。
