Sato M, Yoshida T, Tuboi S
Department of Molecular and Pathological Biochemistry, Yamagata University School of Medicine, Japan.
Biochem Biophys Res Commun. 1989 May 30;161(1):342-7. doi: 10.1016/0006-291x(89)91602-1.
Two cDNA clones for protein carboxyl methyltransferase were isolated from a rat brain cDNA library in lambda gt 11 with synthetic oligonucleotides as probes. The two clones differ in size, but the nucleotide sequence including the whole coding region of the shorter cDNA is completely identical with the corresponding sequence of the longer cDNA. The open reading frame encodes a polypeptide of 227 amino acid residues, with a molecular weight of 24,626. This molecular weight is comparable to those reported for other protein carboxyl methyltransferases from several animals, which were determined by gel filtration chromatography or sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
以合成寡核苷酸为探针，从λgt 11载体的大鼠脑cDNA文库中分离出两个蛋白质羧基甲基转移酶的cDNA克隆。这两个克隆大小不同，但较短cDNA的包括整个编码区的核苷酸序列与较长cDNA的相应序列完全相同。开放阅读框编码一个含227个氨基酸残基的多肽，分子量为24,626。这个分子量与通过凝胶过滤色谱法或十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定的几种动物的其他蛋白质羧基甲基转移酶的分子量相当。
