Wu S V, Chew P, Ho F J, Walsh J H, Wong H, Lee T D, Davis M T, Shively J E, Reeve J R
UCLA School of Medicine, Los Angeles, CA 90024.
Biochem Biophys Res Commun. 1989 May 30;161(1):69-74. doi: 10.1016/0006-291x(89)91561-1.
A peptide identical in structure to the carboxyl-terminal flanking nonapeptide of rat progastrin, predicted by cDNA sequence, was synthesized. The synthetic peptide was used for production of a rabbit antiserum. This antiserum was used to develop a radioimmunoassay specific for rat carboxyl terminal flanking peptide. This assay was used to monitor the purification of immunoreactivity from rat antral extracts. Gel permeation, anion exchange and reverse phase chromatography steps resulted in a single absorbance peak associated with the carboxyl terminal flanking peptide immunoreactivity. The purified peptide eluted in the same position as the synthetic peptide during all three types of chromatography. This material was shown to be identical in mass to Ser-Ala-Glu-Glu-Glu-Asp-Gln-Tyr-Asn, the predicted sequence of the carboxyl terminal nonapeptide of rat progastrin.
通过cDNA序列预测，合成了一种结构与大鼠胃泌素原羧基末端侧翼九肽相同的肽。该合成肽用于制备兔抗血清。此抗血清用于开发一种对大鼠羧基末端侧翼肽具有特异性的放射免疫测定法。该测定法用于监测从大鼠胃窦提取物中免疫反应性物质的纯化过程。凝胶渗透、阴离子交换和反相色谱步骤产生了一个与羧基末端侧翼肽免疫反应性相关的单一吸光度峰。在所有三种类型的色谱中，纯化后的肽与合成肽在相同位置洗脱。该物质的质量被证明与Ser-Ala-Glu-Glu-Glu-Asp-Gln-Tyr-Asn相同，后者是大鼠胃泌素原羧基末端九肽的预测序列。
