Thabet Aouatef, Tlig-Zouari Sabiha, Al Omar Suliman Y, Mansour Lamjed
Research Unit of Integrative Biology and Evolutionary and Functional Ecology of Aquatic Systems, Faculty of Science of Tunis, University of Tunis El Manar, University Campus 2092, Tunis, Tunisia.
Faculty of Sciences of Gabès, University of Gabès, Erriadh City 6072 Zrig, Gabès, Tunisia.
Syst Parasitol. 2016 Jul;93(6):601-11. doi: 10.1007/s11230-016-9647-7. Epub 2016 Jun 15.
During examination of some species of the family Mugilidae, two coelozoic myxozoans were observed in the gall-bladder of the leaping mullet, Liza saliens (Risso). Spore morphology allowed us to allocate them to the genus Ellipsomyxa Køie, 2003, one of which is described here as new. Ellipsomyxa kalthoumi n. sp. was observed forming globular pseudoplasmodia and free spores floating in the bile. Mature spores are ellipsoidal, measuring 13-21 × 10-15 (17.2 × 13.2) µm and possessing two equal spherical polar capsules, 5-6 (5.5) µm in diameter, opening subterminally in opposite directions, with nine polar filament coils. Morphological data and molecular analysis of the small subunit rDNA sequences helped identify this parasite as a new species of Elliposmyxa. The second species identified as E. mugilis (Sitjà-Bobadilla & Alvarez-Pellitero, 1993) has oval spores with rounded ends, measuring 10-11 × 7-9 (10.5 × 8.0) µm and possessing two polar subspherical capsules, 2.7-3.0 (2.8) µm in diameter, opening subterminally in opposite directions. Spore morphometry and molecular study of the small subunit (SSU) of the rRNA gene identified this species as E. mugilis described from the same host in the Western Mediterranean off Spain. Phylogenetic analysis revealed Elliposmyxa as a monophyletic clade and showed that E. mugilis, E. syngnathi Køie & Karlsbakk, 2009, E. adlardi Whipps & Font, 2012 and E. gobii Køie, 2003 group in a subclade containing the Tunisian isolate of E. mugilis whereas E. kalthoumi n. sp. appears in a second subclade together with four Australian species, E. maniliensis Heiniger & Adlard, 2014, E. apogoni Heiniger & Adlard, 2014, E. nigropunctatis Heiniger & Adlard, 2014 and E. arothroni Heiniger & Adlard, 2014.
在对鲻科某些物种进行检查时，在跳鲻（Liza saliens，里索）的胆囊中观察到两种腔寄生粘孢子虫。孢子形态使我们能够将它们归入2003年的Ellipsomyxa属，其中一种在此被描述为新物种。观察到卡尔图米氏艾氏粘体虫（Ellipsomyxa kalthoumi）新种形成球状假原质团和游离孢子漂浮在胆汁中。成熟孢子呈椭圆形，大小为13 - 21×10 - 15（17.2×13.2）微米，具有两个相等的球形极囊，直径5 - 6（5.5）微米，在相反方向亚末端开口，有九个极丝盘绕。小亚基核糖体DNA序列的形态学数据和分子分析有助于将这种寄生虫鉴定为艾氏粘体虫属的一个新物种。第二种被鉴定为鲻艾氏粘体虫（E. mugilis，西贾 - 博巴迪利亚和阿尔瓦雷斯 - 佩利特罗，1993年），其孢子呈椭圆形，两端圆润，大小为10 - 11×7 - 9（10.5×8.0）微米，具有两个亚球形极囊，直径2.7 - 3.0（2.8）微米，在相反方向亚末端开口。孢子形态测量和核糖体RNA基因小亚基（SSU）的分子研究将该物种鉴定为来自西班牙西部地中海同一宿主的鲻艾氏粘体虫。系统发育分析表明艾氏粘体虫是一个单系类群，并显示鲻艾氏粘体虫、2009年的海氏艾氏粘体虫（E. syngnathi）、2012年的阿德氏艾氏粘体虫（E. adlardi）和2003年的戈氏艾氏粘体虫（E. gobii）聚集在一个包含突尼斯分离株鲻艾氏粘体虫的亚类群中，而卡尔图米氏艾氏粘体虫新种与四个澳大利亚物种，即2014年的马尼拉艾氏粘体虫（E. maniliensis）、2014年的无鳍艾氏粘体虫（E. apogoni）、2014年的黑点艾氏粘体虫（E. nigropunctatis）和2014年的阿氏艾氏粘体虫（E. arothroni）一起出现在第二个亚类群中。
