Minakari Mohammad, Golshani Marjan, Yaran Majid, Ataei Behrooz
Department of Infectious Diseases, Isfahan University of Medical Sciences, Isfahan, Iran.
Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2016 May 30;5:90. doi: 10.4103/2277-9175.183138. eCollection 2016.
Hepatitis C infection is one of the most common causes of liver-related morbidity and mortality. Due to limited efficacy and side-effects of treatment, identification of the determinants of response to treatment is an important issue. Nowadays, genotyping of interleukin (IL)-28B is one of the strongest tests used for prediction of sustained virological response. The prevalence of IL28B genotypes varies across different ethnicities. This study presents data on IL28B single nucleotide polymorphism (SNP) (rs12979860) in a group of Iranian hepatitis C virus (HCV)-infected patients in Isfahan.
One hundred patients already diagnosed for hepatitis C enrolled the study. Genomic DNA was extracted from whole blood samples. Specific primers were used to amplify IL28B gene (rs12979860). The rs129679860 SNP was genotyped by real-time polymerase chain reaction using TaqMan(®) probes.
The mean age of patients was 33.16 years (25-42 years). Ninety-nine subjects were male and 1 was female. The frequency of HCV genotypes was as follows: Genotype 3a: 53%, genotype 1a: 42%, genotype 1b: 2%, mixed genotype (1a + 3a): 1% and 2%: Nontypable. IL28B rs12979860 genotypes were TT in 17 patients (17%), CT in 41 patients (41%), and CC in the remaining 42 patients (42%).
The prevalence of C allele is much higher in our population study than in African American HCV patients (62.5% and 40% respectively), which can explain better response to treatment in our patients.
丙型肝炎感染是肝脏相关发病和死亡的最常见原因之一。由于治疗效果有限且存在副作用，确定治疗反应的决定因素是一个重要问题。如今，白细胞介素（IL）-28B基因分型是预测持续病毒学应答最有效的检测方法之一。IL28B基因型的患病率在不同种族中有所不同。本研究展示了一组来自伊斯法罕的伊朗丙型肝炎病毒（HCV）感染患者的IL28B单核苷酸多态性（SNP）（rs12979860）数据。
100例已确诊为丙型肝炎的患者纳入本研究。从全血样本中提取基因组DNA。使用特异性引物扩增IL28B基因（rs12979860）。采用TaqMan®探针通过实时聚合酶链反应对rs129679860 SNP进行基因分型。
患者的平均年龄为33.16岁（25 - 42岁）。99例为男性，1例为女性。HCV基因型频率如下：3a型：53%，1a型：42%，1b型：2%，混合基因型（1a + 3a）：1%，2%：无法分型。IL28B rs12979860基因型中，17例患者为TT（17%），41例患者为CT（41%），其余42例患者为CC（42%）。
在我们的人群研究中，C等位基因的患病率远高于非裔美国HCV患者（分别为62.5%和40%），这可以解释我们的患者对治疗有更好的反应。
