Schmucker Stephane, Sumara Izabela
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC) ; Illkirch, France.
Mol Cell Oncol. 2014 Oct 29;1(2):e954507. doi: 10.1080/23723548.2014.954507. eCollection 2014 Apr-Jun.
Polo-like kinase 1 (PLK1) is a key regulator of eukaryotic cell division. During mitosis, dynamic regulation of PLK1 is crucial for its roles in centrosome maturation, spindle assembly, microtubule-kinetochore attachment, and cytokinesis. Similar to other members of the PLK family, the molecular architecture of PLK1 protein is characterized by 2 domains-the kinase domain and the regulatory substrate-binding domain (polo-box domain)-that cooperate and control PLK1 function during mitosis. Mitotic cells employ many layers of regulation to activate and target PLK1 to different cellular structures in a timely manner. During the last decade, numerous studies have shed light on the precise molecular mechanisms orchestrating the mitotic activity of PLK1 in time and space. This review aims to discuss available data and concepts related to regulation of the molecular dynamics of human PLK1 during mitotic progression.
Polo样激酶1（PLK1）是真核细胞分裂的关键调节因子。在有丝分裂过程中，PLK1的动态调节对于其在中心体成熟、纺锤体组装、微管-动粒附着和胞质分裂中的作用至关重要。与PLK家族的其他成员类似，PLK1蛋白的分子结构由两个结构域——激酶结构域和调节性底物结合结构域（polo框结构域）——组成，这两个结构域在有丝分裂过程中协同作用并控制PLK1的功能。有丝分裂细胞采用多层调节机制，以便及时激活PLK1并将其靶向到不同的细胞结构。在过去十年中，大量研究揭示了在时间和空间上协调PLK1有丝分裂活性的精确分子机制。本综述旨在讨论与有丝分裂进程中人类PLK1分子动力学调节相关的现有数据和概念。
