Chen Yu-Chia, Kilic Ece, Wang Evelyn, Rossman Will, Suzuki Aussie
McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Molecular Cellular Pharmacology Graduate Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.
bioRxiv. 2024 Dec 5:2024.12.05.627000. doi: 10.1101/2024.12.05.627000.
The kinetochore, an intricate macromolecular protein complex located on chromosomes, plays a pivotal role in orchestrating chromosome segregation. It functions as a versatile platform for microtubule assembly, diligently monitors microtubule binding fidelity, and acts as a force coupler. Comprising over 100 distinct proteins, many of which exist in multiple copies, the kinetochore's composition dynamically changes throughout the cell cycle, responding to specific timing and conditions. This dynamicity is important for establishing functional kinetochores, yet the regulatory mechanisms of these dynamics have largely remained elusive. In this study, we employed advanced quantitative immunofluorescence techniques to meticulously chart the dynamics of kinetochore protein levels across the cell cycle. These findings offer a comprehensive view of the dynamic landscape of kinetochore architecture, shedding light on the detailed mechanisms of microtubule interaction and the nuanced characteristics of kinetochore proteins. This study significantly advances our understanding of the molecular coordination underlying chromosome segregation.
动粒是位于染色体上的一种复杂的大分子蛋白质复合体，在协调染色体分离过程中起着关键作用。它作为微管组装的通用平台，勤勉地监测微管结合的保真度，并充当力耦合器。动粒由100多种不同的蛋白质组成，其中许多以多个拷贝存在，其组成在整个细胞周期中动态变化，以响应特定的时间和条件。这种动态性对于建立功能性动粒很重要，然而这些动态变化的调控机制在很大程度上仍然难以捉摸。在这项研究中，我们采用先进的定量免疫荧光技术，精心绘制了整个细胞周期中动粒蛋白水平的动态变化。这些发现提供了动粒结构动态景观的全面视图，揭示了微管相互作用的详细机制以及动粒蛋白的细微特征。这项研究显著推进了我们对染色体分离背后分子协调的理解。
