Barron J T, Kopp S J
Department of Medicine, Rush Medical College, Rush-Presbyterian St. Luke's Medical Center, Chicago, IL 60612.
Biochim Biophys Acta. 1989 Jun 15;1012(1):69-73. doi: 10.1016/0167-4889(89)90012-8.
The purpose of this investigation was to study the effect of fatty acid on carbohydrate metabolism in contracting vascular smooth muscle. The glycogen content of porcine carotid artery incubated in media containing only glucose decreased markedly upon contraction with 80 mM KCl. In contrast, when 0.5 mM palmitate was included there was no decrease in glycogen. Furthermore, the maximal isometric force generated was 22% greater than in the absence of palmitate (P less than 0.001). Stimulation of glycogen phosphorylase a activity with contraction was also enhanced with palmitate. Palmitate had no effect on the levels of the high energy phosphates under any substrate condition. Thus, fatty acid profoundly affects glycogen metabolism and contractility in vascular smooth muscle.
本研究的目的是探讨脂肪酸对收缩期血管平滑肌碳水化合物代谢的影响。在仅含葡萄糖的培养基中孵育的猪颈动脉，用80 mM氯化钾收缩后糖原含量显著降低。相比之下，当加入0.5 mM棕榈酸时，糖原没有减少。此外，产生的最大等长力比未加棕榈酸时大22%（P<0.001）。棕榈酸也增强了收缩对糖原磷酸化酶a活性的刺激作用。在任何底物条件下，棕榈酸对高能磷酸盐水平均无影响。因此，脂肪酸对血管平滑肌的糖原代谢和收缩性有深远影响。
