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核仁素的表达影响微管动力学。

Expression of Nucleolin Affects Microtubule Dynamics.

作者信息

Gaume Xavier, Place Christophe, Delage Helene, Mongelard Fabien, Monier Karine, Bouvet Philippe

机构信息

Université de Lyon, Ecole normale Supérieure de Lyon, Centre de Recherche en Cancérologie de Lyon, Cancer Cell Plasticity Department, UMR INSERM 1052 CNRS 5286, Centre Léon Bérard, Lyon, France.

Université de Lyon, Ecole Normale Supérieure de Lyon, Université Claude Bernard, CNRS, Laboratoire de Physique, F-69342, Lyon, France.

出版信息

PLoS One. 2016 Jun 16;11(6):e0157534. doi: 10.1371/journal.pone.0157534. eCollection 2016.

DOI:10.1371/journal.pone.0157534
PMID:27309529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4911123/
Abstract

Nucleolin is present in diverse cellular compartments and is involved in a variety of cellular processes from nucleolar structure and function to intracellular trafficking, cell adhesion and migration. Recently, nucleolin has been localized at the mature centriole where it is involved in microtubule nucleation and anchoring. Although this new function of nucleolin linked to microtubule regulation has been identified, the global effects of nucleolin on microtubule dynamics have not been addressed yet. In the present study, we analyzed the roles of nucleolin protein levels on global microtubule dynamics by tracking the EB3 microtubule plus end binding protein in live cells. We have found that during microtubule growth phases, nucleolin affects both the speed and life time of polymerization and by analyzing catastrophe events, we showed that nucleolin reduces catastrophe frequency. This new property of nucleolin was then confirmed in a cold induced microtubule depolymerization experiment in which we have found that cold resistant microtubules were totally destabilized in nucleolin depleted cells. Altogether, our data demonstrate a new function of nucleolin on microtubule stabilization, thus bringing novel insights into understanding the multifunctional properties of nucleolin in healthy and cancer cells.

摘要

核仁素存在于多种细胞区室中，参与从核仁结构与功能到细胞内运输、细胞黏附与迁移等多种细胞过程。最近，核仁素定位于成熟中心粒，在那里它参与微管成核和锚定。尽管核仁素与微管调节相关的这一新功能已被确定，但核仁素对微管动力学的整体影响尚未得到研究。在本研究中，我们通过在活细胞中追踪EB3微管正端结合蛋白，分析了核仁素蛋白水平对整体微管动力学的作用。我们发现，在微管生长阶段，核仁素影响聚合的速度和持续时间，并且通过分析解聚事件，我们表明核仁素降低了解聚频率。然后，在冷诱导微管解聚实验中证实了核仁素的这一新特性，我们发现在核仁素缺失的细胞中，抗冷微管完全不稳定。总之，我们的数据证明了核仁素在微管稳定方面的新功能，从而为理解核仁素在健康细胞和癌细胞中的多功能特性带来了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/32b961725292/pone.0157534.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/fcc47e79cc8e/pone.0157534.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/83b4ea3847cc/pone.0157534.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/3bf77f1f596f/pone.0157534.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/972126be4465/pone.0157534.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/2f62086a6b8e/pone.0157534.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/b21608b4dfe6/pone.0157534.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/32b961725292/pone.0157534.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/fcc47e79cc8e/pone.0157534.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/4911123/3700b451b7b1/pone.0157534.g002.jpg
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本文引用的文献

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2
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Cell Cycle. 2015;14(6):902-19. doi: 10.1080/15384101.2014.1000197.
3
Identification of nucleolin as a lipid-raft-dependent β1-integrin-interacting protein in A375 cell migration.鉴定核仁素为脂质筏依赖的β1 整合素相互作用蛋白在 A375 细胞迁移中的作用。
低表达核仁素的 HeLa 细胞中 mRNA 和 miRNA 表达的综合分析。
Sci Rep. 2017 Aug 21;7(1):9017. doi: 10.1038/s41598-017-09353-4.
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Tumor suppressor NF2/Merlin is a microtubule stabilizer.肿瘤抑制因子 NF2/Merlin 是一种微管稳定剂。
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Cell surface nucleolin as a target for anti-cancer therapies.细胞表面核仁素作为抗癌疗法的靶点。
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Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.细胞周期对微管相互作用组的调控：多层次的调控对于有丝分裂/间期的转变至关重要。
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