Shah Forum S, Li Jie, Zanata Fabiana, Curley J Lowry, Martin Elizabeth C, Wu Xiying, Dietrich Marilyn, Devireddy Ram V, Wade James W, Gimble Jeffrey M
LaCell LLC, Tulane University, New Orleans, La., USA.
Cells Tissues Organs. 2015;201(6):436-444. doi: 10.1159/000446494. Epub 2016 Jun 17.
The capability of multipotent mesenchymal stem cells to maintain cell viability, phenotype and differentiation ability upon thawing is critical if they are to be banked and used for future therapeutic purposes. In the present study, we examined the effect of 9-10 months of cryostorage on the morphology, immunophenotype, colony-forming unit (CFU) and differentiation capacity of fresh and cryopreserved human adipose-derived stromal/stem cells (ASCs) from the same donors. Cryopreservation did not reduce the CFU frequency and the expression levels of CD29, CD73, CD90 and CD105 remained unchanged with the exception of CD34 and CD45; however, the differentiation capacity of cryopreserved ASCs relative to fresh cells was significantly reduced. While our findings suggest that future studies are warranted to improve cryopreservation methods and agents, cryopreserved ASCs retain sufficient features to ensure their practical utility for both research and clinical applications.
如果多能间充质干细胞要进行储存并用于未来的治疗目的，那么它们在解冻后维持细胞活力、表型和分化能力的能力至关重要。在本研究中，我们检测了9至10个月的冷冻保存对来自相同供体的新鲜和冷冻保存的人脂肪来源的基质/干细胞（ASC）的形态、免疫表型、集落形成单位（CFU）和分化能力的影响。冷冻保存并未降低CFU频率，除CD34和CD45外，CD29、CD73、CD90和CD105的表达水平保持不变；然而，相对于新鲜细胞，冷冻保存的ASC的分化能力显著降低。虽然我们的研究结果表明有必要开展进一步研究以改进冷冻保存方法和试剂，但冷冻保存的ASC仍保留了足够的特性，以确保其在研究和临床应用中的实际效用。
