Tozzi-Ciancarelli M G, D'Alfonso A, Tozzi E, Troiani-Sevi E, De Matteis G
Cell Mol Biol. 1989;35(2):113-20.
The most important purpose of this research is to characterize by means of fluorescence polarization the structural and functional changes which occur in the membrane of the human erythrocytes during aging process. Our results provide evidence of a significant increase of membrane fluidity in the deep lipid core and in the lipid/protein boundary, in the aged erythrocytes. These features are associated with a rigidity of the membrane surface, as revealed by the anisotropy increase of a specific probe suitable for monitoring the membrane protein behaviour. These modifications could be considered as one of the mechanisms which contribute to alter erythrocyte rheological properties sufficiently to be recognised and removed within circulation.
本研究的最重要目的是通过荧光偏振来表征人类红细胞膜在衰老过程中发生的结构和功能变化。我们的结果表明，衰老红细胞的深层脂质核心和脂质/蛋白质边界处的膜流动性显著增加。这些特征与膜表面的刚性相关，这是通过一种适合监测膜蛋白行为的特定探针的各向异性增加所揭示的。这些修饰可被视为导致红细胞流变特性发生充分改变从而在循环中被识别和清除的机制之一。
