高渗培养基对人细胞生长的影响：III. 子宫内膜异位症（EUE）细胞的细胞动力学变化
Effect of hypertonic medium on human cell growth: III. Changes in cell kinetics of EUE cells.
作者信息
Pellicciari C, Mazzini G, Fuhrman Conti A M, De Grada L, Manfredi Romanini M G
机构信息
Dipartimento di Biologia Animale, C.N.R., Pavia, Italy.
出版信息
Cell Biol Int Rep. 1989 Apr;13(4):345-56. doi: 10.1016/0309-1651(89)90161-6.
The effects of hypertonicity on cell kinetics of EUE cells in culture have been investigated. After 4 days of growth in a hypertonic medium, the plating efficiency of EUE cells was reduced and cell growth was significantly slowed. Flow cytometric measurements of DNA content in synchronized cells, as well as flow cytometric determinations of DNA content and bromodeoxyuridine incorporation in asynchronous cells, also showed that the cell cycle is slowed in a hypertonic medium. In addition, the fraction of cycling cells is smaller and their progression through the S phase slower than in an isotonic medium.
已经研究了高渗对培养的EUE细胞的细胞动力学的影响。在高渗培养基中生长4天后，EUE细胞的接种效率降低，细胞生长显著减慢。对同步化细胞的DNA含量进行流式细胞术测量，以及对非同步化细胞的DNA含量和溴脱氧尿苷掺入进行流式细胞术测定，也表明在高渗培养基中细胞周期减慢。此外，与等渗培养基相比，处于细胞周期的细胞比例更小，它们通过S期的进程更慢。
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