Sivapragasam Magaret, Moniruzzaman Muhammad, Goto Masahiro
Centre of Research in Ionic Liquids (CORIL), Chemical Engineering Department, Universiti Teknologi PETRONAS, Bandar Seri Iskandar, Malaysia.
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
Biotechnol J. 2016 Aug;11(8):1000-13. doi: 10.1002/biot.201500603. Epub 2016 Jun 17.
The technological utility of biomolecules (e.g. proteins, enzymes and DNA) can be significantly enhanced by combining them with ionic liquids (ILs) - potentially attractive "green" and "designer" solvents - rather than using in conventional organic solvents or water. In recent years, ILs have been used as solvents, cosolvents, and reagents for biocatalysis, biotransformation, protein preservation and stabilization, DNA solubilization and stabilization, and other biomolecule-based applications. Using ILs can dramatically enhance the structural and chemical stability of proteins, DNA, and enzymes. This article reviews the recent technological developments of ILs in protein-, enzyme-, and DNA-based applications. We discuss the different routes to increase biomolecule stability and activity in ILs, and the design of biomolecule-friendly ILs that can dissolve biomolecules with minimum alteration to their structure. This information will be helpful to design IL-based processes in biotechnology and the biological sciences that can serve as novel and selective processes for enzymatic reactions, protein and DNA stability, and other biomolecule-based applications.
通过将生物分子（如蛋白质、酶和DNA）与离子液体（ILs）相结合，而非使用传统有机溶剂或水，其技术效用可得到显著提高。离子液体是具有潜在吸引力的“绿色”和“定制”溶剂。近年来，离子液体已被用作生物催化、生物转化、蛋白质保存与稳定、DNA增溶与稳定以及其他基于生物分子应用的溶剂、助溶剂和试剂。使用离子液体可显著提高蛋白质、DNA和酶的结构和化学稳定性。本文综述了离子液体在基于蛋白质、酶和DNA应用方面的最新技术进展。我们讨论了提高生物分子在离子液体中稳定性和活性的不同途径，以及能够以最小程度改变其结构来溶解生物分子的亲生物分子离子液体的设计。这些信息将有助于设计生物技术和生物科学中基于离子液体的工艺，这些工艺可作为酶促反应、蛋白质和DNA稳定性以及其他基于生物分子应用的新型选择性工艺。
