Hu Xuanyu, Guo Wei, Chen Shanshan, Xu Yizhuo, Li Ping, Wang Huaqi, Chu Heying, Li Juan, DU Yuwen, Chen Xiaonan, Zhang Guojun, Zhao Guoqiang
Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
Department of Microbiology and Immunology, Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Oncol Lett. 2016 Jun;11(6):3735-3742. doi: 10.3892/ol.2016.4451. Epub 2016 Apr 18.
Activating enhancer-binding protein (AP)-4 is a member of the basic helix-loop-helix transcription factors, and is involved in tumor biology. However, the role of AP-4 in human lung cancer remains to be fully elucidated. In the present study, the expression of AP-4 in human lung cancer tissues and cells was investigated by reverse transcription-quantitative polymerase chain reaction, and it was observed that the level of AP-4 was increased in tumor tissues and cells compared with their normal counterparts. AP-4 expression was knocked down by transfection with a specific small interfering RNA (siRNA) in lung cancer cells, and this indicated that siRNA-mediated silencing of AP-4 inhibited cell proliferation, arrested the cell cycle at the G0/G1 phase and induced apoptosis by modulating the expression of p21 and cyclin D1. The results of the present study suggest that AP-4 may be an oncoprotein that has a significant role in lung cancer, and that siRNA-mediated silencing of AP-4 may have therapeutic potential as a strategy for the treatment of lung cancer.
激活增强子结合蛋白（AP）-4是碱性螺旋-环-螺旋转录因子家族的成员，参与肿瘤生物学过程。然而，AP-4在人类肺癌中的作用仍有待充分阐明。在本研究中，通过逆转录-定量聚合酶链反应检测了AP-4在人肺癌组织和细胞中的表达，观察到与正常组织和细胞相比，肿瘤组织和细胞中AP-4的水平升高。通过转染特异性小干扰RNA（siRNA）敲低肺癌细胞中AP-4的表达，这表明siRNA介导的AP-4沉默通过调节p21和细胞周期蛋白D1的表达抑制细胞增殖，使细胞周期停滞在G0/G1期并诱导细胞凋亡。本研究结果表明，AP-4可能是一种在肺癌中起重要作用的癌蛋白，并且siRNA介导的AP-4沉默作为一种肺癌治疗策略可能具有治疗潜力。
