Yoo In Seol, Lee Sun Young, Park Chan Keol, Lee Jeong Chan, Kim Young, Yoo Su Jin, Shim Seung Cheol, Choi Yoon Seok, Lee Young, Kang Seong Wook
Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon, Korea.
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
Int J Rheum Dis. 2018 Jan;21(1):148-154. doi: 10.1111/1756-185X.12892. Epub 2016 Jun 17.
There is growing evidence that cold-inducible RNA-binding protein (CIRP) promotes inflammatory responses. This study investigated the relationship between CIRP and rheumatoid arthritis (RA).
Peripheral blood and synovial fluid were collected from 15 patients with RA and from 16 patients with osteoarthritis (OA). The concentration of CIRP was measured with the sandwich enzyme-linked immunosorbent assay (ELISA).
The concentration of serum CIRP was significantly elevated in the RA patient group (RA patients = 26.39 ± 10.48 pg/mL, OA patients = 17.14 ± 7.24 pg/mL, P = 0.009). Furthermore, the RA patient group had a significantly higher CIRP concentration than that of the OA patient group in synovial fluid (153.56 ± 108.93 pg/mL vs. 23.63 ± 16.18 pg/mL, P < 0.001). The mean synovial fluid concentration of CIRP was significantly higher than that of the serum concentration in the RA patient group (serum concentration = 26.39 ± 10.48 pg/mL, synovial fluid = 153.56 ± 108.93 pg/mL, P < 0.001). Disease Activity Score of 28 joints (DAS28)-ESR (erythrocyte sedimentation rate) and DAS28-CRP (C-reactive protein) were positively correlated with the synovial fluid concentration of CIRP (DAS28-ESR: r = 0.582, P = 0.023; DAS28-CRP: r = 0.541, P = 0.037).
The serum and synovial concentrations of CIRP in the RA patients were increased compared to the OA patients. Additionally, the synovial concentration of CIRP in RA patients correlated well with disease activity, that is, the DAS28-ESR/CRP. Based on these results, CIRP mediates inflammation and is a potential marker for synovial inflammation.
越来越多的证据表明冷诱导RNA结合蛋白（CIRP）可促进炎症反应。本研究调查了CIRP与类风湿性关节炎（RA）之间的关系。
收集15例RA患者和16例骨关节炎（OA）患者的外周血和滑液。采用夹心酶联免疫吸附测定（ELISA）法检测CIRP的浓度。
RA患者组血清CIRP浓度显著升高（RA患者=26.39±10.48 pg/mL，OA患者=17.14±7.24 pg/mL，P = 0.009）。此外，RA患者组滑液中CIRP浓度显著高于OA患者组（153.56±108.93 pg/mL对23.63±16.18 pg/mL，P<0.001）。RA患者组滑液中CIRP的平均浓度显著高于血清浓度（血清浓度=26.39±10.48 pg/mL，滑液=153.56±108.93 pg/mL，P<0.001）。28个关节疾病活动评分（DAS28）-红细胞沉降率（ESR）和DAS28- C反应蛋白（CRP）与滑液中CIRP浓度呈正相关（DAS28-ESR：r = 0.582，P = 0.023；DAS28-CRP：r = 0.541，P = 0.037）。
与OA患者相比，RA患者血清和滑液中CIRP浓度升高。此外，RA患者滑液中CIRP浓度与疾病活动度（即DAS28-ESR/CRP）密切相关。基于这些结果，CIRP介导炎症，是滑膜炎症的潜在标志物。
