Paredes-Páliz Karina I, Pajuelo Eloísa, Doukkali Bouchra, Caviedes Miguel Ángel, Rodríguez-Llorente Ignacio D, Mateos-Naranjo Enrique
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, c/Profesor García González, 2, 41012 Sevilla, Spain.
Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, 1095, 41012 Sevilla, Spain.
Mar Pollut Bull. 2016 Sep 15;110(1):396-400. doi: 10.1016/j.marpolbul.2016.06.036. Epub 2016 Jun 14.
The design of effective phytoremediation programs is severely hindered by poor seed germination on metal polluted soils. The possibility that inoculation with plant growth promoting rhizobacteria (PGPR) could help overcoming this problem is hypothesized. Our aim was investigating the role of PGPR in Spartina densiflora seed germination on sediments with different physicochemical characteristics and metal pollution degrees. Gram negative Pantoea agglomerans RSO6 and RSO7, and gram positive Bacillus aryabhattai RSO25, together with the consortium of the three strains, were used for independent inoculation experiments. The presence of metals (As, Cu, Pb and Zn) in sediments reduced seed germination by 80%. Inoculation with Bacillus aryabhattai RSO25 or Pantoea agglomerans RSO6 and RSO7 enhanced up to 2.5 fold the germination rate of S. densiflora in polluted sediments regarding non-inoculated controls. Moreover, the germination process was accelerated and the germination period was extended. The consortium did not achieve further improvements in seed germination.
金属污染土壤上种子发芽率低严重阻碍了有效的植物修复计划的设计。据此推测，接种促生根际细菌（PGPR）可能有助于克服这一问题。我们的目的是研究PGPR在不同理化特性和金属污染程度的沉积物上对互花米草种子萌发的作用。革兰氏阴性聚团泛菌RSO6和RSO7，以及革兰氏阳性阿氏芽孢杆菌RSO25，连同这三种菌株的组合，用于独立的接种实验。沉积物中金属（砷、铜、铅和锌）的存在使种子发芽率降低了80%。与未接种的对照相比，接种阿氏芽孢杆菌RSO25或聚团泛菌RSO6和RSO7可使污染沉积物中互花米草的发芽率提高2.5倍。此外，发芽过程加快，发芽期延长。三种菌株的组合在种子发芽方面没有取得进一步的改善。
