Bensaid M, Malecaze F, Prats H, Bayard F, Tauber J P
INSERM U 168, Laboratoire d'Endocrinologie Expérimentale, CHU Rangueil, Université Paul Sabatier, Toulouse, France.
Exp Eye Res. 1989 Jun;48(6):801-13. doi: 10.1016/0014-4835(89)90065-1.
Fibroblast growth factors (FGFs) are mitogenic for bovine retinal capillary endothelial cells (BREC) seeded at a low density. Seeding BREC cells at a high density greatly reduces their requirement for basic FGF (bFGF) in order to proliferate actively. We show here that monolayers of BREC cells synthesize and release into the culture medium a growth factor, which on the basis of biological activity, heparin affinity, immuno-cross reactivity with anti-bFGF antibodies and mRNA analysis, has been identified as basic fibroblast growth factor. These data indicate that BREC cells are able to synthesize and release bFGF, which can act as a promoting-growth factor for these cells by a para- and/or autocrine mechanism. We suggest thus, that this para- and/or autocrine mechanism involving bFGF may play a key role in preretinal neovascularization, particularly in diabetic patients presenting a proliferative retinopathy.
成纤维细胞生长因子（FGFs）对低密度接种的牛视网膜毛细血管内皮细胞（BREC）具有促有丝分裂作用。高密度接种BREC细胞可大大降低其为实现活跃增殖对碱性成纤维细胞生长因子（bFGF）的需求。我们在此表明，BREC细胞单层可合成一种生长因子并释放到培养基中，基于其生物学活性、肝素亲和力、与抗bFGF抗体的免疫交叉反应性及mRNA分析，该生长因子已被鉴定为碱性成纤维细胞生长因子。这些数据表明，BREC细胞能够合成并释放bFGF，其可通过旁分泌和/或自分泌机制作为这些细胞的促生长因子。因此我们认为，这种涉及bFGF的旁分泌和/或自分泌机制可能在视网膜前新生血管形成中起关键作用，尤其是在患有增殖性视网膜病变的糖尿病患者中。
