Bartó Endre, Prauda Ibolya, Kilár Ferenc, Kiss Ibolya, Felinger Attila
Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Ifjúság útja 6, H-7624 Pécs, Hungary.
Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Ifjúság útja 6, H-7624 Pécs, Hungary.
J Chromatogr A. 2016 Jul 22;1456:152-61. doi: 10.1016/j.chroma.2016.06.002. Epub 2016 Jun 2.
Cavitands are cavity-shaped cyclic oligomers and they can create host-guest interactions with various analytes, therefore they have applications in supramolecular chemistry, nanoscale reactions, chromatographic separations, drug encapsulation and delivery, biochemistry. The investigation of the chromatographic behavior of large molecules, such as resorcinarenes and cavitands is meager up to now. To understand the retention of resorcinarenes and cavitands in liquid chromatography, we studied their retention mechanism by the thermodynamic parameters calculated from the van't Hoff equation and by generation of an adsorption isotherm, which can describe the adsorption of the solute on the stationary phase surface. We compared the thermodynamics of the retention for cyclic oligomers in acetonitrile:water and methanol:water mobile phases. Furthermore, we determined the equilibrium adsorption isotherm by inverse method and we made an error analysis of the estimation obtained with the inverse method to ascertain the validity of the obtained isotherm parameters over a broader concentration range.
穴状配体是呈空腔形状的环状低聚物，它们能与各种分析物形成主客体相互作用，因此在超分子化学、纳米级反应、色谱分离、药物封装与递送、生物化学等领域有应用。到目前为止，对诸如间苯二酚芳烃和穴状配体等大分子的色谱行为研究较少。为了理解间苯二酚芳烃和穴状配体在液相色谱中的保留情况，我们通过由范特霍夫方程计算得到的热力学参数以及通过生成能描述溶质在固定相表面吸附情况的吸附等温线来研究它们的保留机制。我们比较了环状低聚物在乙腈:水和甲醇:水流动相中的保留热力学。此外，我们用反演法确定了平衡吸附等温线，并对用反演法得到的估计值进行了误差分析，以确定在更宽浓度范围内所得到的等温线参数的有效性。
