Dow M P, Bavister B D
Department of Veterinary Science, University of Wisconsin, Madison 53706.
Gamete Res. 1989 Jun;23(2):171-80. doi: 10.1002/mrd.1120230204.
A dialysis unit was used to test whether direct physical contact between serum albumin and hamster spermatozoa is required for capacitation and/or the acrosome reaction. Sperm and bovine serum albumin (BSA) were incubated either together (direct incubation) or separated by a dialysis membrane (indirect incubation). Sperm viability was supported with "sperm motility factors" (hypotaurine and epinephrine) and polyvinylalcohol (PVA). Spermatozoa became capacitated and underwent acrosome reactions when directly incubated in medium containing BSA (TALP-PVA), but did not undergo acrosome reactions when indirectly incubated with BSA (medium TLP-PVA). When sperm were first incubated for 4 hr indirectly with BSA, followed by 4 hr direct incubation with BSA, capacitation did not occur during indirect incubation. These findings indicate that an "intimate association" is necessary between serum albumin and spermatozoa to support capacitation under in vitro incubation conditions. The data are consistent with the concept of direct transfer of compounds from sperm to albumin and/or vice versa during sperm capacitation.
使用透析装置来测试血清白蛋白与仓鼠精子之间的直接物理接触对于获能和/或顶体反应是否必要。精子与牛血清白蛋白（BSA）一起孵育（直接孵育）或通过透析膜分隔孵育（间接孵育）。用“精子活力因子”（亚牛磺酸和肾上腺素）和聚乙烯醇（PVA）维持精子活力。精子在含有BSA的培养基（TALP-PVA）中直接孵育时会发生获能并经历顶体反应，但与BSA间接孵育时（培养基TLP-PVA）不会发生顶体反应。当精子首先与BSA间接孵育4小时，然后再与BSA直接孵育4小时时，间接孵育期间不会发生获能。这些发现表明，在体外孵育条件下，血清白蛋白与精子之间的“紧密关联”对于支持获能是必要的。这些数据与精子获能过程中化合物从精子直接转移到白蛋白和/或反之亦然的概念一致。
