Hazelhoff Roelfzema W, Hacker H J, Van Noorden C J
Laboratory of Cell Biology and Histology, University of Amsterdam, Academic Medical Centre, The Netherlands.
Histochemistry. 1989;91(4):305-8. doi: 10.1007/BF00493005.
Glycogen phosphorylase (PHO) activity was demonstrated histochemically in unfixed cryostat sections of placentae from cadmium-treated and control rats with the use of the semipermeable membrane technique. Staining of the newly synthesized glycogen was performed by lugol. A high activity was present in glycogen cells, spongiotrophoblast and visceral yolk sac from cadmium-treated and control animals. A low but distinct activity could be demonstrated in placental labyrinth from control rats in late pregnancy. Cadmium-exposed rats showed a considerably higher activity in the labyrinth during this period of pregnancy. The elevated PHO activity and concomitant higher glycogen content indicate a disturbance by exposure to cadmium of placental carbohydrate metabolism from day 18 onwards.
利用半透膜技术，通过组织化学方法在镉处理大鼠和对照大鼠胎盘的未固定低温恒温器切片中证实了糖原磷酸化酶（PHO）的活性。新合成糖原的染色用卢戈氏碘液进行。镉处理动物和对照动物的糖原细胞、海绵滋养层和内脏卵黄囊中存在高活性。在妊娠晚期的对照大鼠的胎盘迷路中可显示出低但明显的活性。在此妊娠期间，镉暴露大鼠的迷路中活性明显更高。PHO活性升高以及随之而来的更高糖原含量表明，从第18天起，镉暴露会干扰胎盘碳水化合物代谢。
