羧基血红蛋白部分结合配体物种的动力学研究：（α1CO-β1CO）α2β2 或（α2COβ2CO）α1β1
Kinetic studies on partially liganded species of carboxyhemoglobin: (alpha 1CO-beta 1CO)alpha 2 beta 2 or (alpha 2CO beta 2CO)alpha 1 beta 1.
作者信息
Sharma V S
机构信息
Department of Medicine, University of California, San Diego, La Jolla 92093.
出版信息
J Biol Chem. 1989 Jun 25;264(18):10582-8.
Kinetics of CO combination with and dissociation from isomer III, (alpha 1CO beta 1CO)alpha 2 beta 2 or alpha 1 beta 1 (alpha 2CO beta 2CO), and Hb Rothschild have been studied using the double mixing and microperoxidase methods. Isomer III was prepared in a manner so that it was the only reactive species in the reaction mixture. The biphasic reaction time course in both the "on" and "off" reactions of isomer III and the CO combination reaction of Hb Rothschild are attributed to slow relaxation between the fast and slow CO-reacting species in the two proteins: isomer III: l'f = 6 x 10(6) M-1 s-1, l'dimer = 1.7 x 10(6) M-1 s-1, l's = 2.2 x 10(5) M-1 s-1, lf = 0.15 s-1, ls = 0.01 s-1; Hb Rothschild: l'f = 2.8 x 10(6) M-1 s-1; l's = 2.7 x 10(5) M-1 s-1.
已使用双混合法和微过氧化物酶法研究了一氧化碳与异构体III（α1COβ1CO）α2β2或α1β1（α2COβ2CO）以及血红蛋白罗斯柴尔德（Hb Rothschild）的结合和解离动力学。异构体III的制备方式使其成为反应混合物中唯一的反应性物种。异构体III的“结合”和“解离”反应以及Hb Rothschild的CO结合反应中的双相反应时间进程归因于两种蛋白质中快速和慢速CO反应物种之间的缓慢弛豫：异构体III：l'f = 6×10⁶ M⁻¹ s⁻¹，l二聚体 = 1.7×10⁶ M⁻¹ s⁻¹，l's = 2.2×10⁵ M⁻¹ s⁻¹，lf = 0.15 s⁻¹，ls = 0.01 s⁻¹；Hb Rothschild：l'f = 2.8×10⁶ M⁻¹ s⁻¹；l's = 2.7×10⁵ M⁻¹ s⁻¹。
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