环核苷酸门控通道18作为拟南芥花粉萌发和花粉管生长必需的钙通道发挥作用。
Cyclic nucleotide-gated channel 18 functions as an essential Ca channel for pollen germination and pollen tube growth in Arabidopsis.
作者信息
Gu Li-Li, Gao Qi-Fei, Wang Yong-Fei
机构信息
a National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences , Chinese Academy of Sciences , Shanghai , China.
b University of Chinese Academy of Sciences , Shanghai , China.
出版信息
Plant Signal Behav. 2017 Nov 2;12(11):e1197999. doi: 10.1080/15592324.2016.1197999. Epub 2016 Jun 20.
We recently revealed that cyclic nucleotide-gated channel 18 (CNGC18) functioned as the main Ca channel in pollen tube tips for pollen tube guidance to ovules by regulating external Ca influx in Arabidopsis. In this study, we found that the reduction of external Ca concentration ([Ca]) from 10 mM to 5 mM, and further to 2 mM, led to the decreases of pollen germination percentages, but led to the increases of the percentages of ruptured pollen grains and tubes, and branched pollen tubes in vitro in cngc18-17 compared with wild type. The second point mutant allele cngc18-22 showed similar phenotypes, including reduced pollen germination percentages, increased percentages of ruptured pollen tubes, but did not show obvious different percentages of ruptured pollen grains and branched pollen tubes compared with wild type. These data demonstrate that CNGC18 plays essential roles in pollen germination and tube growth as a Ca channel in Arabidopsis.
我们最近发现，在拟南芥中，环核苷酸门控通道18（CNGC18）作为花粉管顶端的主要钙离子通道，通过调节外部钙离子内流来引导花粉管向胚珠生长。在本研究中，我们发现，将外部钙离子浓度（[Ca]）从10 mM降至5 mM，再降至2 mM，会导致花粉萌发率降低，但与野生型相比，cngc18 - 17突变体在体外培养时，破裂花粉粒和花粉管的比例以及花粉管分支增加。第二个点突变等位基因cngc18 - 22表现出类似的表型，包括花粉萌发率降低、破裂花粉管比例增加，但与野生型相比，破裂花粉粒和花粉管分支的比例没有明显差异。这些数据表明，在拟南芥中，CNGC18作为钙离子通道在花粉萌发和花粉管生长中起着至关重要的作用。
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