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三种 CNGC 家族成员（CNGC5、CNGC6 和 CNGC9）作为钙渗透性通道对于根毛的组成性生长是必需的。

Three CNGC Family Members, CNGC5, CNGC6, and CNGC9, Are Required for Constitutive Growth of Root Hairs as Ca-Permeable Channels.

机构信息

National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.

University of Chinese Academy of Sciences, Shanghai 200032, China.

出版信息

Plant Commun. 2019 Sep 16;1(1):100001. doi: 10.1016/j.xplc.2019.100001. eCollection 2020 Jan 13.

DOI:10.1016/j.xplc.2019.100001
PMID:33404548
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7748020/
Abstract

The genetic identities of Ca channels in root hair (RH) tips essential for constitutive RH growth have remained elusive for decades. Here, we report the identification and characterization of three cyclic nucleotide-gated channel (CNGC) family members, CNGC5, CNGC6, and CNGC9, as Ca channels essential for constitutive RH growth in . We found that the triple mutant (designated ) showed significantly shorter and branching RH phenotypes as compared with the wild type. The defective RH growth phenotype of could be rescued by either the expression of , , or single gene or by the supply of high external Ca, but could not be rescued by external K supply. Cytosolic Ca imaging and patch-clamp data in HEK293T cells showed that these three CNGCs all function as Ca-permeable channels. Cytosolic Ca imaging in growing RHs further showed that the Ca gradients and their oscillation in RH tips were dramatically attenuated in compared with those in the wild type. Phenotypic analysis revealed that these three CNGCs are Ca channels essential for constitutive RH growth, with different roles in RHs from the conditional player CNGC14. Moreover, we found that these three CNGCs are involved in auxin signaling in RHs. Taken together, our study identified CNGC5, CNGC6, and CNGC9 as three key Ca channels essential for constitutive RH growth and auxin signaling in .

摘要

几十年来，对于构成根毛（RH）尖端组成性 RH 生长所必需的 Ca 通道的遗传身份一直难以捉摸。在这里，我们报告了三种环核苷酸门控通道（CNGC）家族成员 CNGC5、CNGC6 和 CNGC9 的鉴定和特征，它们是 中构成性 RH 生长所必需的 Ca 通道。我们发现，与野生型相比，三突变体（命名为 ）表现出明显较短和分支的 RH 表型。 的缺陷 RH 生长表型可以通过表达单个基因 、 、 或 ，或通过提供高外部 Ca 来挽救，但不能通过外部 K 供应来挽救。HEK293T 细胞中的细胞质 Ca 成像和膜片钳数据表明，这三种 CNGC 均作为 Ca 通透通道发挥作用。在生长中的 RH 中的细胞质 Ca 成像进一步表明，与野生型相比， 在 RH 尖端的 Ca 梯度及其振荡显着减弱。表型分析表明，这三种 CNGC 是构成性 RH 生长所必需的 Ca 通道，在 RH 中具有与条件性 CNGC14 不同的作用。此外，我们发现这三种 CNGC 参与了 RH 中的生长素信号转导。总之，我们的研究确定了 CNGC5、CNGC6 和 CNGC9 作为构成性 RH 生长和生长素信号转导所必需的三个关键 Ca 通道。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/e882cc46099b/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/25625828d03f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/6774b59c1155/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/5b1f0abb4a6e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/ced3ee6d1687/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/6f0bc17c1d5d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/8a687e50526f/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/e882cc46099b/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/25625828d03f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/6774b59c1155/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/5b1f0abb4a6e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/ced3ee6d1687/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/6f0bc17c1d5d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/8a687e50526f/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b9d/7748020/e882cc46099b/gr7.jpg

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