Qin L M, Qin G M, Shi X H, Wang A L, Zuo H
The First Department of Cardiovascular Internal Medicine, Xianyang Central Hospital of Shaanxi, Xianyang, China.
Department of Digestive Internal Medicine, The First People's Hospital of Xianyang, Xianyang, China.
Genet Mol Res. 2016 May 13;15(2):gmr7632. doi: 10.4238/gmr.15027632.
We conducted a case-control study to investigate the role of one single nucleotide polymorphism of MMP-9 rs3918242 in the development of coronary artery disease. The rs3918242 was amplified with 435-bp DNA fragments using polymerase chain reaction coupled with restriction fragment length polymorphism. When compared with control subjects, patients with coronary artery disease had higher systolic and diastolic blood pressure, as well as higher triglycerides (P < 0.05), were more likely to suffer from diabetes mellitus, and had lower total cholesterol and high-density lipopolysaccharides. Using unconditional logistic analysis, we found that individuals with CT and TT genotypes were associated with increased risk of coronary artery disease in a co-dominant model, and the ORs (95%CI) were 1.50 (1.02-2.20) and 6.89 (2.51-23.41) for CT and TT, respectively. We observed that the T allele of rs3918242 was correlated with increased risk of coronary artery disease (OR = 1.88, 95%CI = 1.39-2.55). In conclusion, we suggest that the TT and CT genotypes and T allele of MMP-9 rs3918242 polymorphism is correlated with an increased risk of coronary artery disease in a Chinese population.
我们进行了一项病例对照研究，以调查基质金属蛋白酶-9（MMP-9）基因rs3918242的单核苷酸多态性在冠状动脉疾病发生中的作用。采用聚合酶链反应结合限制性片段长度多态性技术，扩增出435bp的DNA片段以检测rs3918242。与对照组相比，冠状动脉疾病患者的收缩压和舒张压更高，甘油三酯水平也更高（P<0.05），更易患糖尿病，总胆固醇和高密度脂蛋白水平更低。通过非条件逻辑回归分析，我们发现在共显性模型中，CT和TT基因型个体患冠状动脉疾病的风险增加，CT和TT基因型的比值比（OR）（95%可信区间）分别为1.50（1.02 - 2.20）和6.89（2.51 - 23.41）。我们观察到rs3918242的T等位基因与冠状动脉疾病风险增加相关（OR = 1.88，95%可信区间 = 1.39 - 2.55）。总之，我们认为MMP-9 rs3918242多态性的TT和CT基因型以及T等位基因与中国人群冠状动脉疾病风险增加相关。
