Zhu X H, Cheng S P, Liao T, Kang X Y
National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Laboratory of Urban and Rural Ecological Environment, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
College of Forestry and Horticulture, Xinjiang Agricultural University, East Nongda Road, Urumqi, China.
Genet Mol Res. 2016 May 25;15(2):gmr7899. doi: 10.4238/gmr.15027899.
Populus talassica Kom. is an ecologically important species endemic to central Asia. In China, its main distribution is restricted to the Ili region in the Xinjiang Autonomous Region. An understanding of genetic diversity and population structure is crucial for the development of a feasible conservation strategy. Twenty-six high-level simple sequence repeat (SSR) markers were screened and used to genotype 220 individuals from three native populations. A high level of genetic diversity and low population differentiation were revealed. We identified 163 alleles, with a mean of 6.269 alleles per locus. The observed and expected heterozygosities ranged from 0.472 to 0.485 (with a mean of 0.477), and from 0.548 to 0.591 (mean 0.569), respectively. Analysis of molecular variance revealed 93% variation within populations and 7% among populations. A model-based population structure analysis divided P. talassica into two groups (optimal K = 2). These genetic data provide crucial insight for conservation management.
胡杨是中亚特有的具有重要生态意义的物种。在中国，其主要分布局限于新疆维吾尔自治区的伊犁地区。了解遗传多样性和种群结构对于制定可行的保护策略至关重要。筛选了26个高级简单序列重复（SSR）标记，并用于对来自三个本地种群的220个个体进行基因分型。结果显示出高水平的遗传多样性和低种群分化。我们鉴定出163个等位基因，每个位点平均有6.269个等位基因。观察到的杂合度和预期杂合度分别在0.472至0.485之间（平均为0.477）和0.548至0.591之间（平均为0.569）。分子方差分析表明，93%的变异存在于种群内部，7%的变异存在于种群之间。基于模型的种群结构分析将胡杨分为两组（最佳K = 2）。这些遗传数据为保护管理提供了关键的见解。
