Cole Christopher T
Division of Science and Mathematics, University of Minnesota-Morris, 600 E. 4th St., Morris, MN 56267, USA.
New Phytol. 2005 Jul;167(1):155-64. doi: 10.1111/j.1469-8137.2005.01423.x.
To develop a robust basis for inferences about population genetics and evolution, this work assayed 192 aspens (Populus tremuloides) from 11 sites in Wisconsin, USA, for allelic and population variation at 16 microsatellite loci distributed across the Populus genome. Frequency distributions of fluorochrome-labeled alleles resolved by capillary electrophoresis were analyzed for relationships to repeat size and number. Population-level statistics were compared with those of other studies, especially in Populus. All loci were polymorphic, varying widely in the number of alleles per locus (mean = 8.25, range 2-20). Expected and observed heterozygosities were high (0.45 and 0.41, respectively), with little differentiation among populations (F(ST) = 0.006-0.045) and a moderate level of inbreeding (F(IS) = 0.09), intermediate among levels reported in studies based on isozymes. Contrary to several other reports, allele frequencies clustered tightly around the modal frequency, and the genetic diversity (measured as alleles per locus or as expected heterozygosity) was not related to either the repeat unit size or to the number of repeats.
为了建立有关群体遗传学和进化推断的坚实基础，本研究对来自美国威斯康星州11个地点的192株颤杨（Populus tremuloides）进行了分析，检测了分布在杨树基因组中的16个微卫星位点的等位基因和群体变异。分析了通过毛细管电泳解析的荧光染料标记等位基因的频率分布与重复序列大小和数量的关系。将群体水平的统计数据与其他研究，特别是杨树研究的数据进行了比较。所有位点均为多态性，每个位点的等位基因数量差异很大（平均值 = 8.25，范围2 - 20）。预期杂合度和观察到的杂合度都很高（分别为0.45和0.41），群体间分化很小（F(ST) = 0.006 - 0.045），近亲繁殖水平适中（F(IS) = 0.09），在基于同工酶的研究报告的水平中处于中等。与其他几份报告相反，等位基因频率紧密聚集在模态频率周围，并且遗传多样性（以每个位点的等位基因数或预期杂合度衡量）与重复单元大小或重复次数均无关。
