Sousa Ana Rita Sequeira de, Staff Anne Cathrine, Johnsen Guro Mørk, Weedon-Fekjær Mina Susanne, Størvold Gro Leite
Department of Obstetrics and Department of Gynecology, Oslo University Hospital, Ullevål, Norway; Faculty of Medicine, University of Oslo, Norway; Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.
Department of Obstetrics and Department of Gynecology, Oslo University Hospital, Ullevål, Norway; Faculty of Medicine, University of Oslo, Norway.
Placenta. 2016 Jul;43:9-12. doi: 10.1016/j.placenta.2016.04.009. Epub 2016 Apr 26.
Reverse transcription quantitative PCR (RT-qPCR) gene expression results must be normalized using stably expressed genes to correct for technical variation. We evaluated the expression of four widely used normalizers (RNA18S, GAPDH, TBP, and YWHAZ) across 59 decidual tissue samples collected by vacuum suction from preeclamptic and normotensive pregnancies. RNA18S and GAPDH were not suitable as normalizers, while YWHAZ and TBP were stably expressed across the study groups.
逆转录定量聚合酶链反应（RT-qPCR）基因表达结果必须使用稳定表达的基因进行标准化，以校正技术差异。我们评估了四种广泛使用的标准化基因（RNA18S、甘油醛-3-磷酸脱氢酶（GAPDH）、TATA盒结合蛋白（TBP）和14-3-3ζ蛋白（YWHAZ））在通过真空抽吸收集的59份子痫前期和血压正常孕妇的蜕膜组织样本中的表达情况。RNA18S和GAPDH不适合作为标准化基因，而YWHAZ和TBP在各研究组中均稳定表达。
