Tornvall P, Carlson L A
King Gustaf V Research Institute, Karolinska Institute, Stockholm, Sweden.
J Intern Med. 1989 May;225(5):359-61. doi: 10.1111/j.1365-2796.1989.tb00096.x.
Isolated, small very low density lipoproteins (VLDL) from males with plasma concentrations of VLDL triglycerides ranging between 0.92 and 8.65 mmol l-1 were incubated with lipoprotein lipase. There were no differences in the size of the VLDL particles isolated from normo-respectively hypertriglyceridaemic plasmas. After incubation, 4.5 +/- 3.5% (mean +/- SD) of the initial glyceride-glycerol remained in the incubate. This amount did not vary with the plasma VLDL concentration. The incubation mixture was separated into three density fractions: VLDL, low density lipoprotein (LDL) and a high density lipoprotein (HDL) respectively. The lipid contents of these were determined. The distribution of cholesterol and phospholipids after lipolysis was highly dependent on the plasma VLDL level. The amount of cholesterol and phospholipids recovered in the LDL density region increased with the rising plasma VLDL level, the correlation coefficients for this relationship were 0.80 and 0.66, respectively. The results thus indicate that small, similar size VLDL from normo-respectively hypertriglyceridaemic plasmas have different properties.
从血浆中极低密度脂蛋白（VLDL）甘油三酯浓度在0.92至8.65 mmol l-1之间的男性中分离出的小的极低密度脂蛋白（VLDL）与脂蛋白脂肪酶一起孵育。从正常和高甘油三酯血症血浆中分离出的VLDL颗粒大小没有差异。孵育后，孵育物中仍保留4.5±3.5%（平均值±标准差）的初始甘油酯甘油。该量不随血浆VLDL浓度而变化。将孵育混合物分离为三个密度级分：分别为VLDL、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）。测定了它们的脂质含量。脂解后胆固醇和磷脂的分布高度依赖于血浆VLDL水平。在LDL密度区域回收的胆固醇和磷脂量随着血浆VLDL水平的升高而增加，这种关系的相关系数分别为0.80和0.66。因此，结果表明，来自正常和高甘油三酯血症血浆的大小相似的小VLDL具有不同的性质。
