Piedra P A, Camussi G, Ogra P L
Department of Pediatrics, State University of New York, Buffalo.
J Gen Virol. 1989 Feb;70 ( Pt 2):325-33. doi: 10.1099/0022-1317-70-2-325.
Groups of mice were immunized intraperitoneally with HEp-2 lysate or respiratory syncytial virus (RSV)-infected HEp-2 cells with or without adjuvant(s). The animals were subsequently challenged intranasally (i.n.) with RSV and/or HEp-2 lysate and studied for RSV shedding in the lung, antigen-specific antibody response and pulmonary histopathology. A significant decrease in virus shedding was detected in each of four groups of animals immunized with a virus preparation and in two of four groups that received a HEp-2 lysate. Mice immunized with adjuvant(s) developed higher antibody-specific responses. All vaccinated animals developed pulmonary histopathology only on subsequent i.n. inoculation with RSV and/or HEp-2 lysate. Denatured extracts of purified RSV, HEp-2, BALB/c lung, cotton rat lung, Buffalo green monkey kidney and human buccal epithelium were tested for reactivity against the sera of immunized mice by an immunoblot method. Sera from all groups of immunized mice reacted with the extracts tested. The data suggest a possible role of reactivity to viral as well as non-viral components in the pathogenesis of RSV vaccine-induced pulmonary inflammation in the mouse model system.
将小鼠分组，用HEp-2裂解物或呼吸道合胞病毒（RSV）感染的HEp-2细胞腹腔内免疫，添加或不添加佐剂。随后，给动物经鼻内（i.n.）接种RSV和/或HEp-2裂解物，并研究肺内RSV脱落情况、抗原特异性抗体反应和肺组织病理学。在用病毒制剂免疫的四组动物中的每组以及接受HEp-2裂解物的四组中的两组中，均检测到病毒脱落显著减少。用佐剂免疫的小鼠产生了更高的抗体特异性反应。所有接种疫苗的动物仅在随后经鼻内接种RSV和/或HEp-2裂解物时才出现肺组织病理学变化。通过免疫印迹法检测纯化的RSV、HEp-2、BALB/c肺、棉鼠肺、水牛绿猴肾和人颊黏膜上皮的变性提取物与免疫小鼠血清的反应性。所有免疫小鼠组的血清均与所测试的提取物发生反应。数据表明，在小鼠模型系统中，对病毒和非病毒成分的反应性在RSV疫苗诱导的肺部炎症发病机制中可能起作用。
