Yang Hui, He Hao, Tan Bin, Liu Enmei, Zhao Xiaodong, Zhao Yao
Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.
Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.
Mol Cell Probes. 2016 Aug;30(4):231-237. doi: 10.1016/j.mcp.2016.06.003. Epub 2016 Jun 18.
Human metapneumovirus (hMPV) is a prevalent pathogen worldwide and causes various respiratory infections. Although it is a critical pathogen in pediatric patients, it is unclear how it enters host cells. In this study, we focused on hMPV cell entry using two kinds of cell lines (Vero E6 and LLC-MK2), which are most commonly used for isolating and propagating for hMPV, and we used fluorescent dyes to label the virus particles and monitored how they enter the host cell in real time. We found that endocytosis was the predominant pathway by which hMPV entered host cells. When the virus particles were traced inside host cells, we found that a low intracellular pH was needed for intracellular fusion in LLC-MK2 cells.
人偏肺病毒（hMPV）是一种在全球广泛流行的病原体，可引起各种呼吸道感染。尽管它是儿科患者中的关键病原体，但其进入宿主细胞的机制尚不清楚。在本研究中，我们使用两种最常用于分离和培养hMPV的细胞系（Vero E6和LLC-MK2），聚焦于hMPV进入细胞的过程，并使用荧光染料标记病毒颗粒，实时监测它们如何进入宿主细胞。我们发现内吞作用是hMPV进入宿主细胞的主要途径。当在宿主细胞内追踪病毒颗粒时，我们发现在LLC-MK2细胞中，细胞内融合需要较低的细胞内pH值。
