Chen Qifu, Guo Wei, Zhang Ying, Wu Yaochen, Xiang Jin
Department of Neurosurgery, The Third People's Hospital of Shenzhen, Shenzhen, 518112, China.
JiNan University, Second Clinical Medicine College, Shenzhen People's Hospital, Department of Neurosurgery, Shenzhen, 518020, China.
Neurosci Lett. 2016 Aug 15;628:161-6. doi: 10.1016/j.neulet.2016.06.031. Epub 2016 Jun 18.
MicroRNA-19a (miR-19a) is upregulated in different types of cancers, including gliomas, but its specific role and function in gliomas have yet to be fully elucidated. In this study, we found that miR-19a was significantly upregulated in human glioma tissues and cell lines. Overexpression of miR-19a by a miR-19a mimic promoted glioma cell proliferation and invasion. In contrast, miR-19a inhibitor suppressed cell proliferation and invasion. Furthermore, by a dual-luciferase reporter assay and expression analysis, we determined that Ras homolog family member B was a direct target of miR-19a. Knockdown of Ras homolog family member B could block cell proliferation and invasion induced by the miR-19a mimic. In conclusion, our study demonstrated that miR-19a upregulation is common in gliomas and that suppression of miR-19a expression inhibits cell proliferation and invasion, which indicates that miR-19a may act as an oncogene in gliomas.
微小RNA-19a（miR-19a）在包括神经胶质瘤在内的不同类型癌症中表达上调，但其在神经胶质瘤中的具体作用和功能尚未完全阐明。在本研究中，我们发现miR-19a在人神经胶质瘤组织和细胞系中显著上调。通过miR-19a模拟物过表达miR-19a可促进神经胶质瘤细胞增殖和侵袭。相反，miR-19a抑制剂可抑制细胞增殖和侵袭。此外，通过双荧光素酶报告基因检测和表达分析，我们确定Ras同源家族成员B是miR-19a的直接靶点。敲低Ras同源家族成员B可阻断miR-19a模拟物诱导的细胞增殖和侵袭。总之，我们的研究表明miR-19a上调在神经胶质瘤中很常见，抑制miR-19a表达可抑制细胞增殖和侵袭，这表明miR-19a可能在神经胶质瘤中作为癌基因发挥作用。
