Golec Julian R, Duan Jian J, Aparicio Ellen, Hough-Goldstein Judith
Department of Entomology and Wildlife Ecology, University of Delaware, Newark, DE 19713 (
United States Department of Agriculture, Agricultural Research Service, Beneficial Insects Introduction Research Unit, Newark, DE 19713 (
J Econ Entomol. 2016 Aug;109(4):1545-54. doi: 10.1093/jee/tow122. Epub 2016 Jun 21.
The invasive Asian longhorned beetle, Anoplophora glabripennis (Motschulsky), is a destructive xylophagous forest pest species originating from Asia. Several endemic North American hymenopteran (Braconidae) species in the mid-Atlantic region were capable of attacking and reproducing on A. glabripennis larvae in laboratory bioassays. Ontsira mellipes Ashmead (Hymenoptera: Braconidae) has been continually reared on A. glabripennis larvae at USDA-ARS BIIRU since 2010, and has been identified as a potential new-association biocontrol agent. Two experiments were conducted to investigate parasitism, paralysis, reproductive biology, larval development, and longevity of adult O. mellipes In the first experiment, pairs of adult parasitoids were given single A. glabripennis larvae every 2 d (along with honey and water) over their lifetimes, while in the second experiment individual parasitoids were observed daily from egg to adult, and adults were subsequently starved. Adults in the first experiment parasitized ∼21% of beetle larvae presented to them throughout their life, and paralysis of larvae occurred 1-2 d after oviposition. More than half of the individual pairs parasitized A. glabripennis larvae, with each female producing around 26 offspring throughout her life. In the second experiment, median development time of O. mellipes from egg to adult was about 3 wk, with five larval instars. Adult O. mellipes that were provided with host larvae, honey, and water lived 9 d longer than host-deprived and starved adults. These findings indicate that mass-rearing procedures for O. mellipes may be developed using the new association host for development of effective biocontrol programs against A. glabripennis.
入侵性亚洲长角天牛（Anoplophora glabripennis (Motschulsky)）是一种源自亚洲的极具破坏力的蛀木森林害虫。在大西洋中部地区，几种北美本土膜翅目（茧蜂科）物种在实验室生物测定中能够攻击光肩星天牛幼虫并在其上繁殖。自2010年以来，美国农业部农业研究局生物防治入侵昆虫研究室一直在用光肩星天牛幼虫连续饲养梅氏奥姬小蜂（Ontsira mellipes Ashmead，膜翅目：茧蜂科），并且它已被确定为一种潜在的新关联生物防治剂。进行了两项实验，以研究梅氏奥姬小蜂的寄生、麻痹、生殖生物学、幼虫发育和成虫寿命。在第一个实验中，成对的成年寄生蜂在其一生中每2天被给予一只光肩星天牛幼虫（同时提供蜂蜜和水），而在第二个实验中，从卵到成虫每天观察单个寄生蜂，随后成虫饥饿处理。在第一个实验中，成虫在其一生中寄生了约21%提供给它们的甲虫幼虫，幼虫在产卵后1至2天发生麻痹。超过一半的成对个体寄生了光肩星天牛幼虫，每只雌蜂一生大约产生26个后代。在第二个实验中，梅氏奥姬小蜂从卵到成虫的中位发育时间约为3周，有五个幼虫龄期。提供宿主幼虫、蜂蜜和水的成年梅氏奥姬小蜂比没有宿主且饥饿的成年个体寿命长9天。这些发现表明，可以利用这种新关联宿主开发梅氏奥姬小蜂的大量饲养程序，以制定针对光肩星天牛的有效生物防治计划。
