Bayati Vahid, Abbaspour Mohammad Reza, Dehbashi Fereshteh Negad, Neisi Niloofar, Hashemitabar Mahmoud
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Anat Sci Int. 2017 Sep;92(4):509-520. doi: 10.1007/s12565-016-0352-z. Epub 2016 Jun 21.
Polycaprolactone (PCL) is used as a material of choice for surgical sutures, wound dressings, contraceptives, fixation devices and dentistry in paramedical sciences. In addition, adipose-derived stem cells (ASCs) have been shown to be effective in the treatment of acute and chronic wounds. This study aimed to evaluate the effect of electrospun PCL fibers on keratinocyte differentiation of ASCs and wound healing. PCL solution was electrospun and characterized. Isolated and characterized ASCs were differentiated into keratinocyte-like cells on a tissue culture plate (TCP) and PCL matrices and compared. PCL nano-/microfibers cultured with ASCs (test group) or alone (control) were implanted as a dermal substitute for wound healing. There were significant increases in the proliferation rate and expression level of cytokeratin 14, filaggrin and involucrin in cells cultured on PCL matrices compared to TCP (p < 0.05). After histological and immunological evaluation of the reconstituted skin, a thick epidermal layer with several skin appendages was evidently observed in the ASC/PCL group, whereas no real and mature epidermis was formed, especially in the central area of the healing wound in the pure PCL group on day 14. Pure PCL, if possessing suitable properties including good adhesiveness, high proliferative capability, inductive elasticity and stiffness for migration and differentiation, could drive the keratinocyte differentiation of ASCs and act as an efficient dermal equivalent to promote wound healing.
聚己内酯（PCL）被用作辅助医疗科学中手术缝线、伤口敷料、避孕药、固定装置和牙科领域的首选材料。此外，脂肪来源干细胞（ASC）已被证明在急性和慢性伤口治疗中有效。本研究旨在评估电纺PCL纤维对ASC角质形成细胞分化及伤口愈合的影响。对PCL溶液进行电纺并表征。将分离并表征的ASC在组织培养板（TCP）和PCL基质上分化为角质形成细胞样细胞并进行比较。将与ASC共培养的PCL纳米/微纤维（试验组）或单独的PCL纳米/微纤维（对照组）作为真皮替代物植入用于伤口愈合。与TCP相比，在PCL基质上培养的细胞中细胞角蛋白14、细丝聚集蛋白和兜甲蛋白的增殖率和表达水平显著增加（p < 0.05）。对重组皮肤进行组织学和免疫学评估后，在ASC/PCL组明显观察到有几个皮肤附属器的厚表皮层，而在第14天，纯PCL组愈合伤口的中央区域尤其没有形成真正成熟的表皮。如果纯PCL具有合适的特性，包括良好的粘附性、高增殖能力、诱导迁移和分化的弹性和硬度，它可以驱动ASC的角质形成细胞分化，并作为一种有效的真皮替代物促进伤口愈合。
