亚急性职业性铅暴露个体中的谷胱甘肽、谷胱甘肽相关酶与氧化应激
Glutathione, glutathione-related enzymes, and oxidative stress in individuals with subacute occupational exposure to lead.
作者信息
Dobrakowski Michał, Pawlas Natalia, Hudziec Edyta, Kozłowska Agnieszka, Mikołajczyk Agnieszka, Birkner Ewa, Kasperczyk Sławomir
机构信息
Department of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze, Poland.
Institute of Occupational Medicine and Environmental Health in Sosnowiec, ul. Kościelna 13, 41-200 Sosnowiec, Poland.
出版信息
Environ Toxicol Pharmacol. 2016 Jul;45:235-40. doi: 10.1016/j.etap.2016.06.008. Epub 2016 Jun 7.
The aim of the study was to investigate the influence of subacute exposure to lead on the glutathione-related antioxidant defense and oxidative stress parameters in 36 males occupationally exposed to lead for 40±3.2days. Blood lead level in the examined population increased significantly by 359% due to lead exposure. Simultaneously, erythrocyte glutathione level decreased by 16%, whereas the activity of glutathione-6-phosphate dehydrogenase in erythrocytes and leukocytes decreased by 28% and 10%, respectively. Similarly, the activity of glutathione-S-transferase in erythrocytes decreased by 45%. However, the activity of glutathione reductase in erythrocytes and leukocytes increased by 26% and 6%, respectively, whereas the total oxidant status value in leukocytes increased by 37%. Subacute exposure to lead results in glutathione pool depletion and accumulation of lipid peroxidation products; however, it does not cause DNA damage. Besides, subacute exposure to lead modifies the activity of glutathione-related enzymes.
本研究的目的是调查36名职业性接触铅40±3.2天的男性亚急性接触铅对谷胱甘肽相关抗氧化防御和氧化应激参数的影响。由于铅暴露，被检查人群的血铅水平显著升高了359%。同时，红细胞谷胱甘肽水平下降了16%，而红细胞和白细胞中6-磷酸葡萄糖脱氢酶的活性分别下降了28%和10%。同样，红细胞中谷胱甘肽-S-转移酶的活性下降了45%。然而，红细胞和白细胞中谷胱甘肽还原酶的活性分别增加了26%和6%，而白细胞中的总氧化剂状态值增加了37%。亚急性接触铅会导致谷胱甘肽池耗竭和脂质过氧化产物积累；然而，它不会导致DNA损伤。此外，亚急性接触铅会改变谷胱甘肽相关酶的活性。
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