Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India.
Environ Toxicol. 2014 Feb;29(2):138-45. doi: 10.1002/tox.20780. Epub 2011 Oct 19.
In the present study, we have studied the effect of KBrO3 on human erythrocytes under in vitro conditions. Erythrocytes were isolated from the blood of healthy nonsmoking volunteers and incubated with different concentrations of KBrO3 at 37°C for 60 min. This resulted in marked hemolysis in a KBrO3 -concentration dependent manner. Lysates were prepared from KBrO3 -treated and control erythrocytes and assayed for various parameters. KBrO3 treatment caused significant increase in protein oxidation, lipid peroxidation, hydrogen peroxide levels, and decrease in total sulfhydryl content, which indicates induction of oxidative stress in human erythrocytes. Methemoglobin levels and methemoglobin reductase activity were significantly increased while the total antioxidant power of lysates was greatly reduced upon KBrO3 treatment. Intracellular production of reactive oxygen species increased in a dose dependent manner. Exposure of erythrocytes to KBrO3 also caused decrease in the activities of catalase, glutathione peroxidase, thioredoxin reductase, glucose 6-phosphate dehydrogenase and glutathione reductase whereas the activities of Cu-Zn superoxide dismutase and glutathione-S-transferase were increased. These results show that KBrO3 induces oxidative stress in human erythrocytes through the generation of reactive oxygen species and alters the cellular antioxidant defense system.
在本研究中，我们研究了 KBrO3 在体外条件下对人红细胞的影响。红细胞从健康不吸烟志愿者的血液中分离出来，在 37°C 下与不同浓度的 KBrO3 孵育 60 分钟。这导致了 KBrO3 浓度依赖性的明显溶血。从 KBrO3 处理和对照红细胞中制备裂解物，并测定各种参数。KBrO3 处理导致蛋白质氧化、脂质过氧化、过氧化氢水平显著增加，总巯基含量降低，这表明人红细胞中诱导了氧化应激。高铁血红蛋白水平和高铁血红蛋白还原酶活性显著增加，而裂解物的总抗氧化能力大大降低。活性氧的细胞内产生呈剂量依赖性增加。红细胞暴露于 KBrO3 还导致过氧化氢酶、谷胱甘肽过氧化物酶、硫氧还蛋白还原酶、葡萄糖 6-磷酸脱氢酶和谷胱甘肽还原酶的活性降低，而铜锌超氧化物歧化酶和谷胱甘肽-S-转移酶的活性增加。这些结果表明，KBrO3 通过产生活性氧诱导人红细胞的氧化应激，并改变细胞抗氧化防御系统。
