一种基于丹磺酰氯的易于制备的荧光pH探针。
An easily Prepared Fluorescent pH Probe Based on Dansyl.
作者信息
Sha Chunming, Chen Yuhua, Chen Yufen, Xu Dongmei
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, Jiangsu, 215123, China.
Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Soochow University, Suzhou, Jiangsu, 215123, China.
出版信息
J Fluoresc. 2016 Sep;26(5):1709-14. doi: 10.1007/s10895-016-1861-9. Epub 2016 Jun 22.
A novel fluorescent pH probe from dansyl chloride and thiosemicarbazide was easily prepared and fully characterized by (1)H NMR, (13)C NMR, LC-MS, Infrared spectra and elemental analysis. The probe exhibited high selectivity and sensitivity to H(+) with a pK a value of 4.98. The fluorescence intensity at 510 nm quenched 99.5 % when the pH dropped from 10.88 to 1.98. In addition, the dansyl-based probe could respond quickly and reversibly to the pH variation and various common metal ions showed negligible interference. The recognition could be ascribed to the intramolecular charge transfer caused by the protonation of the nitrogen in the dimethylamino group.
一种由丹磺酰氯和氨基硫脲制备的新型荧光pH探针易于制备，并通过¹H NMR、¹³C NMR、LC-MS、红外光谱和元素分析进行了全面表征。该探针对H⁺表现出高选择性和灵敏度，pKa值为4.98。当pH从10.88降至1.98时，510 nm处的荧光强度猝灭了99.5%。此外，基于丹磺酰基的探针可以对pH变化快速且可逆地做出响应，各种常见金属离子的干扰可忽略不计。这种识别可归因于二甲氨基中氮的质子化引起的分子内电荷转移。
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