Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, PR China.
Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, PR China; State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
Biomaterials. 2015;53:669-78. doi: 10.1016/j.biomaterials.2015.02.113. Epub 2015 Mar 24.
Pyridinium functioned 7-hydroxy coumarin was presented as the first mitochondria-targeted ratiometric fluorescent probe CP for real time monitoring pH in living cells. Compared with commercially available mitochondrial trackers, CP possesses high specificity to mitochondria in living cells as well as good biocompatibility. Meanwhile, CP displays excellent pH sensitivity and anti-interference capability. Confocal image experiments confirm that CP can monitor mitochondrial pH changes associated with the mitochondrial acidification, cellular apoptosis and stress response efficiently in real time.
吡啶鎓 7-羟基香豆素被呈现为第一个线粒体靶向比率型荧光探针 CP，用于实时监测活细胞中的 pH 值。与市售的线粒体示踪剂相比，CP 对活细胞中的线粒体具有高特异性和良好的生物相容性。同时，CP 显示出优异的 pH 敏感性和抗干扰能力。共聚焦图像实验证实 CP 能够实时有效地监测与线粒体酸化、细胞凋亡和应激反应相关的线粒体 pH 值变化。
