Bonthius D J, West J R
Department of Anatomy, College of Medicine, University of Iowa 52242.
Neurotoxicol Teratol. 1989 Mar-Apr;11(2):135-43. doi: 10.1016/0892-0362(89)90052-4.
The purpose of this study was to determine whether the microencephaly in rats resulting from early postnatal alcohol exposure is altered by a concurrent administration of aspirin. Neonatal rats were artificially reared from postnatal day 4 to postnatal day 10, a period of the brain growth spurt in the rat that is similar to the third trimester of human brain development. The alcohol-treated groups received 6.6 g/kg/day of ethanol and either 0.0, 12.5, 25.0 or 50.0 mg/kg/day of aspirin in a milk solution. Control groups received either 0.0 (gastrostomy control), 12.5, 25.0 or 50.0 mg/kg/day of aspirin in a milk solution free of alcohol. Brainstem, cerebellum and total brain weights were measured on postnatal day 10. Alcohol alone significantly reduced the mean total brain weight, cerebellum and brainstem weight by 19.8%, 23.1% and 12.2%, respectively, relative to gastrostomy controls. A significant interaction between ethanol and aspirin was observed for total brain weight. The mean total brain weight of the group receiving both alcohol and 50 mg/kg/day aspirin was significantly lower than all other experimental groups and was reduced 29.5%, relative to gastrostomy controls. The highest dose of aspirin alone significantly reduced cerebellar weight, relative to gastrostomy controls but had no effect on brainstem or total brain weight.
本研究的目的是确定出生后早期酒精暴露导致的大鼠小头畸形是否会因同时给予阿司匹林而改变。新生大鼠从出生后第4天人工饲养至出生后第10天，这是大鼠脑发育快速期，类似于人类大脑发育的第三个 trimester。酒精处理组在乳溶液中接受6.6 g/kg/天的乙醇和0.0、12.5、25.0或50.0 mg/kg/天的阿司匹林。对照组在不含酒精的乳溶液中接受0.0（胃造口术对照）、12.5、25.0或50.0 mg/kg/天的阿司匹林。在出生后第10天测量脑干、小脑和全脑重量。相对于胃造口术对照，单独酒精显著降低了平均全脑重量、小脑和脑干重量，分别降低了19.8%、23.1%和12.2%。观察到乙醇和阿司匹林对全脑重量有显著交互作用。同时接受酒精和50 mg/kg/天阿司匹林的组的平均全脑重量显著低于所有其他实验组，相对于胃造口术对照降低了29.5%。单独使用最高剂量的阿司匹林相对于胃造口术对照显著降低了小脑重量，但对脑干或全脑重量没有影响。
