Chalghmi Houssem, Zrafi Ines, Gourves Pierre-Yves, Bourdineaud Jean-Paul, Saidane-Mosbahi Dalila
UMR CNRS 5805 EPOC, University of Bordeaux, Arcachon Marine Station, Place du Dr Peyneau, 33120 Arcachon, France.
Center of Water Researches and Technologies, Technopark Borj Cedria, BP. 273, 8020 Soliman, Tunisia.
Environ Sci Process Impacts. 2016 Jul 13;18(7):895-907. doi: 10.1039/c6em00139d.
The spatial and seasonal alteration in a battery of biomarkers responses (enzymatic activity of glutathione-S-transferase, catalase and acetylcholinesterase and lipid peroxidation) were investigated to assess the metal derived effects in clam (Ruditapes decussatus) gills, collected from Tunis lagoon (Tunisia). Trace metals (Ag, As, Cd, Cu, Hg, Mn, Ni, Pb, V and Zn) concentrations were assessed seasonally in sediments and tissues of R. decussatus from three different sites (S1, S2 and S3). Bioaccumulation factor (BAF) analysis showed a spatio-temporal variation of metal uptake rates in clams through sediments. Likewise, the multibiomarker approach enabled a time-site trend differentiation between sites with distinctive degrees of anthropogenic contamination. Site S2 was identified as the most impacted region due to the presence of different contamination sources (shipping and industrial activities). The results suggest that biomarker's seasonal variation arises from a complex interaction between environmental conditions (e.g. temperature, salinity) and probably biological factors (mainly the reproduction process) along with anthropogenic pressure. The general biological response measured with the IBR index at all the sampling sites revealed the highest metabolic stress in summer. The combined effects of metal contamination and increased temperature and salinity in summer appear to induce the highest metabolic adaptation response. The selected biomarkers provided an integrated response, which is useful for the assessment of the combined effects of metal contamination and abiotic parameters in clams and the environmental status of coastal lagoon ecosystem.
研究了一系列生物标志物反应（谷胱甘肽 - S - 转移酶、过氧化氢酶和乙酰胆碱酯酶的酶活性以及脂质过氧化）的空间和季节变化，以评估从突尼斯泻湖采集的蛤（紫贻贝）鳃中金属衍生的影响。对来自三个不同地点（S1、S2和S3）的紫贻贝沉积物和组织中的痕量金属（银、砷、镉、铜、汞、锰、镍、铅、钒和锌）浓度进行了季节性评估。生物累积因子（BAF）分析表明，蛤通过沉积物吸收金属的速率存在时空变化。同样，多生物标志物方法能够区分具有不同程度人为污染的地点之间的时间 - 地点趋势。由于存在不同的污染源（航运和工业活动），S2地点被确定为受影响最严重的区域。结果表明，生物标志物的季节变化源于环境条件（如温度、盐度）与可能的生物因素（主要是繁殖过程）以及人为压力之间的复杂相互作用。在所有采样地点用IBR指数测量的一般生物反应显示，夏季代谢压力最高。夏季金属污染以及温度和盐度升高的综合影响似乎诱导了最高的代谢适应反应。所选生物标志物提供了综合反应，这对于评估蛤中金属污染和非生物参数的综合影响以及沿海泻湖生态系统的环境状况很有用。
